alexa VEGF Induces IL-23 Expression in Keratinocytes through p38 Signaling
ISSN: 2155-9554

Journal of Clinical & Experimental Dermatology Research
Open Access

Like us on:
OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Research Article

VEGF Induces IL-23 Expression in Keratinocytes through p38 Signaling

Miriam Canavese1,2*, Mark Peric1, Yvonne Dombrowski1, Sarah Koglin1, Thomas Ruzicka1 and Jürgen Schauber1

1Department of Dermatology and Allergy, Ludwig-Maximilian-University, Munich, Germany

2Harvard Medical School & MGH Cancer Center, 185 Cambridge Street, Simches Building, Boston, MA, USA

*Corresponding Author:
Dr. Miriam Canavese
Harvard Medical School & MGH Cancer Center,
185 Cambridge Street, Simches Building, Boston, MA  02114, USA
Tel: 6177244955
Fax: 617 724 2662
E-mail: [email protected]

Received date: October 07, 2011; Accepted date: November 01, 2011; Published date: November 06, 2011

Citation: Canavese M, Peric M, Dombrowski Y, Koglin S, Ruzicka T, et al. (2011) VEGF Induces IL-23 Expression in Keratinocytes through p38 Signaling. J Clin Exp Dermatol Res S2:002. doi:10.4172/2155-9554.S2-002

Copyright: © 2011 Canavese M, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

 

Abstract

Background: Psoriasis is a chronic inflammatory skin disorder which is associated with increased cutaneous vascular endothelial growth factor (VEGF) expression. Several studies demonstrate that VEGF plays an important role in psoriasis pathogenesis by linking angiogenesis and inflammation. We aimed to study the molecular functions of VEGF in psoriasis and dissect a link between VEGF and pro-inflammatory cytokine expression in human keratinocytes.
Methods: VEGF expression was evaluated in sections from lesional psoriatic skin by immunohistochemistry. Cultured keratinocytes were transfected with a VEGF expression plasmid and changes in pro-inflammatory cytokine expression were investigated by qPCR, ELISA and dot-blot. Protein phosphorylation profiles in lysates from cells overexpressing VEGF were analysed to identify the underlying signaling pathways. Specific inhibitors or siRNA knockdown were used in confirmatory experiments.
Results: In lesional psoriatic skin, VEGF and the pro-inflammatory cytokine IL-23 are both strongly expressed by epidermal keratinocytes. VEGF over-expression in cultured keratinocytes resulted in increased IL-23 and IL-6 mRNA transcript abundance and protein expression. At the same time VEGF over-expression strongly increased phosphorylation of p38 MAPK, CREB and HSp27. Inhibition of p38 MAPK by SB203580 blocked VEGF induced IL-23 expression while siRNA mediated knockdown of CREB or HSp27 showed no effect.
Conclusions: VEGF up-regulates pro-inflammatory IL-23 and IL-6 secretion through p38 MAPK in epidermal keratinocytes in psoriasis. Targeting VEGF and/or p38 MAPK could lead to novel anti-inflammatory treatments for this chronic skin disease.

Share This Page

Additional Info

Loading
Loading Please wait..
 
Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri, Food, Aqua and Veterinary Science Journals

Dr. Krish

[email protected]

1-702-714-7001 Extn: 9040

Clinical and Biochemistry Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals

Ronald

[email protected]

1-702-714-7001Extn: 9042

Chemical Engineering and Chemistry Journals

Gabriel Shaw

[email protected]

1-702-714-7001 Extn: 9040

Earth & Environmental Sciences

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

Engineering Journals

James Franklin

[email protected]

1-702-714-7001Extn: 9042

General Science and Health care Journals

Andrea Jason

[email protected]

1-702-714-7001Extn: 9043

Genetics and Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001 Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Informatics Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Material Sciences Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Mathematics and Physics Journals

Jim Willison

[email protected]

1-702-714-7001 Extn: 9042

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001 Extn: 9038

Neuroscience & Psychology Journals

Nathan T

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

John Behannon

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001 Extn: 9042

 
© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
adwords