Wet Extraction of Lipids and Astaxanthin from Haematococcus pluvialis by Liquefied Dimethyl EtherPanatpong Boonnoun1,2, Yuko Kurita1, Yuichi Kamo1, Wahyudiono1, Siti Machmudah1,3, Yuji Okita4, Eiji Ohashi4, Hideki Kanda1,5* and Motonobu Goto1
- *Corresponding Author:
- Hideki Kanda
Department of Chemical Engineering
Nagoya University, Chikusa, Nagoya 464-8603, Japan
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Received date: July 17, 2014; Accepted date: August 25, 2014; Published date: August 27, 2014
Citation: Boonnoun P, Kurita Y, Kamo Y, Wahyudiono, Machmudah S, et al. (2014) Wet Extraction of Lipids and Astaxanthin from Haematococcus pluvialis by Liquefied Dimethyl Ether. J Nutr Food Sci 4:305. doi: 10.4172/2155-9600.1000305
Copyright: © 2014 Boonnoun P, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Recently, a simple method for the extraction of lipids from wet cyanobacterial microalgae using liquefied dimethyl ether (DME) without drying, cell disruption, or heating was proposed. Herein, the versatility of this method was evaluated using Haematococcus pluvialis at 0.59 MPa and 25°C. Direct extraction of lipids from H. pluvialis of moisture-rich microalgae was successfully achieved, in a lipid extraction yield of 30.0% of the dry weight of the microalgae. The astaxanthin concentration in the extracted lipid was 0.33%, which was lower than the 1.82% achieved by the commonly-used acetone extraction, which incorporates drying and cell disruption. The carbon and hydrogen content were improved after DME extraction. In addition, 92% of water in the wet H. pluvialis was removed. Compared with other extractions, liquefied DME combines drying, cell description, and solvent extraction into one step.