Journal of Aquaculture Research & Development
Open Access

White Shrimp Litopenaeus vannamei that have received Petalonia binghamiae Extract Activate Immunity, Increase Immune Response and Resistance against Vibrio alginolyticus

Chen YY¹, Chen JC^1*, Lin YC¹, Yeh ST¹, Chao KP² and Lee CS¹

¹Department of Aquaculture, National Taiwan Ocean University, Keelung, Taiwan

²Department of Cosmeceutics, China Medical University, Taichung, Taiwan

*Corresponding Author:

Chen JC
Department of Aquaculture
College of Life Sciences
National Taiwan Ocean University
Keelung, Taiwan
Tel: +886-2-2462- 0295
Fax: +886-2-2462-0295
E-mail: [email protected]

Received Date: August 13, 2014; Accepted Date: September 18, 2014; Published Date: September 28, 2014

Citation: Chen YY, Chen JC, Lin YC, Yeh ST, Chao KP, et al. (2014) White Shrimp Litopenaeus vannamei that have received Petalonia binghamiae Extract Activate Immunity, Increase Immune Response and Resistance against Vibrio alginolyticus. J Aquac Res Development 5:268. doi:10.4172/2155-9546.1000268

Copyright: 2014 Chen YY, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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This study was to examine immune response of white shrimp Litopenaeus vannamei and its resistance against Vibrio alginolyticus when shrimp received Petalonia binghamiae extract in vitro and in vivo. Shrimp hemocytes incubated with 1 mg/ml extract showed increased Phenoloxidase (PO) activity and respiratory burst (RB, release of superoxide anion). The total hemocyte count (THC), Phenoloxidase (PO) activity, respiratory Burst (RB) of shrimp received the extract at 6 and 10 μg/g were significantly higher than those of shrimp received saline and the control shrimp after 48, 96 and 144 h. Superoxide Dismutase (SOD) activity of shrimp received the extract at 6 and 10 μg/g were significantly higher than those of shrimp received saline and the control shrimp after 48 h. Phagocytic activity and clearance efficiency to Vibrio alginolyticus in the shrimp that received the extract at 6 and 10 μg/g were significantly higher than those of shrimp received saline and the control shrimp after 24, 48, 96 and 144 h. In another experiment, shrimp which had received the extract at 2, 6, and 10 μg/g after one day were challenged with V. alginolyticus at 1.4×106 colony-forming units (cfu)/shrimp, and then placed in seawater. The survival rate of shrimp that received the extract at 6 and 10 μg/g was significantly higher than that of control shrimp after 12-144 h. It was concluded that P. binghamiae extract could cause activation of shrimp immunity, and concluded that shrimp received the extract at 6~10 μg/g increased immune response of shrimp as well as resistance to V. alginolyticus infection.