Xenotransplantation Transgenesis. Are we there yet?
|Nottle MB1*, Hawthorne W2, O’Connell PJ2, d’Apice AJFM3 and Cowan PJ3|
|1Reproductive Biotechnology Group, Centre for Stem Cell Research, Robinson Institute, School of Paediatrics and Reproductive Health, University of Adelaide, Adelaide South Australia, 5005 Australia|
|2The Centre for Transplant and Renal Research, Westmead Millennium Research Institute, University of Sydney, Westmead, NSW, 2145, Australia|
|3Immunology Research Centre, St Vincent’s Hospital & Department of Medicine, University of Melbourne, Melbourne, 3000 Victoria, Australia|
|Corresponding Author :||B. Nottle
Reproductive Biotechnology Group, Centre for Stem Cell Research
Robinson Institute School of Paediatrics and Reproductive Health
University of Adelaide, Adelaide, South Australia, 5005, Australia
Tel: 61+ 8 8313 4087
Fax : 61+ 8 8313 4099
E-mail: [email protected] au
|Received: April 20, 2015 Accepted: May 29, 2015 Published: June 01, 2015|
|Citation:Nottle MB, Hawthorne W, O’Connell PJ, d’Apice AJF, Cowan PJ (2015) Xenotransplantation Transgenesis. Are we there yet? Clon Transgen 4: 137. doi:10.4172/2168-9849.1000137|
|Copyright: ©2015 Nottle MB, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.|
The xenotransplantation of pig organs and tissues aimed at overcoming the world-wide shortage that exists for these in humans will require multiple genetic modifications to be incorporated to overcome rejection and associated problems. We have recently produced α1,3 galactosyltransferase knockout (GTKO) pigs expressing human (h) CD55&59, fucosyltransferase (HTF), hCD39 and hTBM This was done by breeding GTKO pigs to animals expressing human hCD55 and hCD59 plus HTF and then transfecting adult fibroblasts obtained from their progeny with a hCD39 plus hTBM construct. Transfected cells were then selected based on hCD39 expression and used for animal cloning to produce these (Figure 1). The generation of these animals represents almost two decades of research focussed on developing the necessary molecular, cellular and reproductive technologies. Having produced GT KO pigs expressing five transgenes, it is an appropriate juncture to reflect on how this was achieved and what progress still needs to be made in terms of transgenesis technology to advance xenotransplantation to the clinic.