alexa Zebrafish in the Wild: Microhabitat Use by Zebrafish Da
ISSN: 2150-3508

Fisheries and Aquaculture Journal
Open Access

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Research Article

Zebrafish in the Wild: Microhabitat Use by Zebrafish Danio rerio (Hamilton, 1822) from Karala River of Jalpaiguri District, Northern Bengal, India

Manickam Raja, Ramalingam Karthik Raja and Pachiappan Perumal*

Department of Biotechnology, Periyar University, Periyar Palkalai Nagar, Salem, Tamil Nadu

*Corresponding Author:
P Perumal
Professor, Head, Department of Biotechnology
Periyar University, Periyar Palkalai Nagar
Salem 636 011, Tamil Nadu, India
Tel: +91 427 2345766x225
Fax: +91 427 2345124
E-mail: [email protected]

Received date: August 19, 2016; Accepted date: December 01, 2016; Published date: December 10, 2016

Citation: Raja M, Raja RK, Perumal P (2016) Zebrafish in the Wild: Microhabitat Use by Zebrafish Danio rerio (Hamilton, 1822) from Karala River of Jalpaiguri District, Northern Bengal, India. Fish Aqua J 7:179. doi:10.4172/2150-3508.1000179

Copyright: © 2016 Raja M, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.



The microhabitat preference of zebrafish, Danio rerio from Karala river was investigated. The availability/ preference of microhabitat variables such as (i) distance from the stream bank (ii) water column depth (iii) mesohabitat (iv) water velocity (v) substratum and (vi) subaquatic vegetal cover were quantified based on underwater observations covering a total of 400 m2 of stream area. Microhabitat selectivity was analyzed by comparing the microhabitat availability in the study site and the microhabitat used by fish. Data availability and microhabitats usage pattern were used for calculating habitat availability/preference of D. rerio. In order to test the microhabitat preference of D. rerio the Principal Component Analysis (PCA) and Canonical Correspondence Analysis (CCA) were applied. In PCA the first three components with higher Eigenvalue accounted for about 98.46% of the total variance. The first component axis alone explained 86.33% of the variance with an Eigenvalue of 5.3 with high loadings (>0.7). CCA revealed a clear separation of the D. rerio along the microhabitat variables. Among the thirty two microhabitat variables, the forward selection procedure was found to be positively significant with eighteen microhabitat variables P<0.05. The preference of D. rerio in the studied streams viz: pool, plunge pool and isolated pool; slow flow, moderate flow and fast flow; and the substratum parameters: sand, gravel and leaf litter; depth parameters like Depth 1, Depth 2, Depth 3 and Depth 5; fish cover parameters such as root undercut and distance from bank parameters such as Distance from bank 1, Distance from bank 2 and Distance from bank 3. CCA revealed a clear distribution pattern of the D. rerio along the microhabitat variables.


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