|"Two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) has been widely used for proteomic investigations of C. albicans. 2D-PAGE has typically been used for protein separation for proteome analysis. The shotgun approach is an alternative strategy for proteomic analysis that combines LC-MS/MS. This method can identify many kinds of proteins, including a small quantity of proteins in a high-through put manner. In addition, systems with ultra- performance chromatographic separation have been constructed, and have demonstrated excellent performance.
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Citation: Shibasaki S, Karasaki M, Ueda M (2014) Combining Proteomic Strategies and Molecular Display Technology for Development of Vaccines against Candida albicans. J Proteomics Bioinform 7: 134-138."