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Population Genetics for 15 STR loci of Liaoning Han in Northeastern China | OMICS International
ISSN: 2157-7145
Journal of Forensic Research

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Population Genetics for 15 STR loci of Liaoning Han in Northeastern China

Yongji Zhang*

Department of Forensic Medicine, Yanbian University College of Medicine, Yanji City, Jilin Province, China

*Corresponding Author:
Dr. Yongji Zhang
Department of Forensic Medicine
Yanbian University College of Medicine
977 gongyuan road, 133002 Yanji City
Jilin Province, P.R. China
E-mail: [email protected]

Received date: March 10, 2011; Accepted date: April 07, 2011; Published date: April 11, 2011

Citation: Zhang Y (2011) Population Genetics for 15 STR loci of Liaoning Han in Northeastern China. J Forensic Res 2:123. doi: 10.4172/2157-7145.1000123

Copyright: © 2011 Zhang Y. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Abstract

Allele frequencies for the 15 autosomal STR loci D3S1358, TH01, D21S11, D18S51. Penta E, D5S818, D13S317, D7S820, D16S539, CSF1PO, Penta D, vWA, D8S1179, TPOX, and FGA (PowerPlex ® 16 system ™ , Promega corporation)were determined in a population sample of 538 unrelated individuals from northeastern China Han (Liaoning).

Keywords

STR; Allele frequencies; Northeastern China Han; PowerPlex ® 16 system

Population

Blood samples were collected from 538 unrelated individuals from the northeastern China Han(Liaoning).

Extraction

DNA was extracted from whole blood using the Chelex-100 extraction method [1].

PCR

PCR amplification was performed using 1.0 ng genomic DNA template according to manufacturer's protocol (PowerPlex® 16 system, Promega Corporation) [2].

Typing

PCR products were separated on an ABI Prism 3100 Genetic Analyzer (Applied Biosystems) using the recommended protocol. The results were analyzed by Data Collection (Version 1.1), GeneScan (Version 3.7), and Genotyper (Version 3.6) softwares (Applied Biosystems).

Analysis of Data

Allele frequencies and summary statistics were calculated with "PowerStats V 12" p rogram (Promega home page:http://www. promega.com/geneticidtools/). Estimations of Hardy-Weinberg equilibrium according to Guo and Thompson [3]. To compare our data to those from other group samples, Arlequin 2.0 was used to calculate pairwise diffrencese for each locus [4].

Quality control

Laboratory Internal Control Standards and Kit controls.

Access to the data

Via electronic mail from communicating author: [email protected] edu.cn.

Results

Allele frequencies for each locus are presented in Table 1. Forensic statistics for the 15 loci are summarized in Table 2.

  D3S1358 TH01 D21S11 D18S51 Penta E D5S818 D13S317 D7S820 D16S539 CSF1PO Penta D vWA D8S1179 TPOX FGA
Allele n=538 n=538 n=538 n=538 n=538 n=538 n=538 n=538 n=538 n=538 n=538 n=538 n=538 n=538 n=538
5         0.0428                    
6   0.1050                 0.0028        
7   0.2416     0.0019 0.0093 0.0065     0.0019 0.0056     0.0009  
8   0.0530     0.0037 0.0009 0.2630 0.1292 0.0065 0.0009 0.0436   0.0009 0.5325  
9   0.5437     0.0084 0.0594 0.1227 0.0799 0.2890 0.0604 0.2931   0.0009 0.1125  
9.3   0.0325                          
10   0.0214   0.0019 0.0502 0.2189 0.1543 0.1599 0.1171 0.2128 0.1252   0.0836 0.0251  
11   0.0028   0.0037 0.1199 0.3219 0.2388 0.3522 0.2574 0.2342 0.1753   0.0846 0.3011  
12       0.0381 0.0948 0.2347 0.1599 0.2435 0.2100 0.3931 0.1670   0.1375 0.0270  
13       0.1896 0.0455 0.1391 0.0372 0.0316 0.1041 0.0855 0.1494   0.2184 0.0009  
14 0.0417     0.2165 0.0781 0.0102 0.0177 0.0037 0.0149 0.0102 0.0306 0.2649 0.1868    
15 0.3813     0.1719 0.1152 0.0037     0.0009 0.0009 0.0056 0.0344 0.1812    
16 0.3284     0.1301 0.0976 0.0009         0.0009 0.1691 0.0883    
17 0.1837     0.0641 0.0939             0.2305 0.0139   0.0009
18 0.0622     0.0595 0.0818 0.0009         0.0009 0.1989 0.0037   0.0270
19 0.0028     0.0362 0.0586             0.0836     0.0465
20       0.0279 0.0586             0.0158     0.0437
20.2                             0.0019
21       0.0279 0.0242             0.0028     0.1059
21.2                             0.0028
22       0.0186 0.0112                   0.1320
22.2                             0.0084
22.3                             0.0019
23       0.0093 0.0084                   0.2212
23.2                             0.0065
24       0.0046 0.0019                   0.2138
24.2                             0.0056
25         0.0037                   0.0967
25.2                             0.0037
26                             0.0576
26.2                             0.0019
27                             0.0186
28     0.0409                       0.0037
28.2     0.0046                        
29     0.2463                        
29.2     0.0009                        
30     0.3132                        
30.2     0.0130                        
30.3     0.0056                        
31     0.1022                        
31.2     0.0595                        
32     0.0297                        
32.2     0.1264                        
33     0.0084                        
33.2     0.0437                        
34     0.0028                        
34.2     0.0009                        
35     0.0009                        
35.2     0.0009                        

Table 1: STR allele frequencies of 538 individuals from northeastern China(Liao Ning) Han.

  D3S1358 TH01 D21S11 D18S51 Penta E D5S818 D13S317 D7S820 D16S539 CSF1PO Penta D vWA D8S1179 TPOX FGA
He 0.7075 0.6307 0.8065 0.8582 0.9199 0.7703 0.8076 0.7670 0.7813 0.7342 0.8146 0.8001 0.8435 0.6117 0.8588
Ho 0.6902 0.6450 0.7881 0.8662 0.9349 0.7774 0.8011 0.7602 0.7770 0.7435 0.7885 0.7714 0.8383 0.6059 0.8606
PIC 0.6558 0.5852 0.7813 0.8428 0.9121 0.7340 0.7801 0.7332 0.7470 0.6926 0.7900 0.7703 0.8241 0.5499 0.8438
PE 0.4132 0.3484 0.5772 0.7270 0.8672 0.5577 0.6011 0.5274 0.5570 0.4987 0.5779 0.5470 0.6719 0.2981 0.7159
PD 0.8657 0.8229 0.9392 0.9632 0.9852 0.9081 0.9348 0.9118 0.9126 0.8868 0.9400 0.9311 0.9556 0.7912 0.9636
P 0.6977 0.7612 0.2924 0.7479 0.0987 0.7173 0.7893 0.9609 0.1495 0.3675 0.1520 0.9558 0.1595 0.9817 0.2945

Table 2: Statistical parameters of the 15 STR loci for forensic interest in northeastern China(Liao Ning) Han.

Other Remarks

No significant deviation from Hardy-Weinberg expectations (p> 0.05) for 15 STR loci. A comparative analysis between our data and previously published data for the same loci has been done using the statistical method of pairwise differentiation test. The comparative results showed that northeastern China (Liaoning) Han population has significant differences from Caucasian at 10 locus (except D3S1358, D18S51, Penta D, D8S1179 and FGA) [5], African at 13 locus (except D3S1358 and D16S539) [6], respectively. And there were no significant difference between the studied population and Beijing Han [7], Sichuan Han (except D18S51) [8], Hongkong Han (except TH01 and D18S51) [9], Southeastern Han [10], Northwestern Han (except D18S51) [11], South Korean (except TH01) [12], and Japanese (except TH01) [13,14]. The combined MP (Matching Probability) and PE (power of exclusion) for 15 STR loci were 5.76×10-18 and 0.999998, respectively.

This paper follows the guidelines for publication of population data requested by the journal [15].

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