2Viral Diseases Division, National Microbiology Laboratory, Public Health Agency of Canada, Canadian Science Centre for Human and Animal Health, 1015 Arlington St., Winnipeg, Manitoba, R3E 3R2, Canada
Received Date: October 12, 2011; Accepted Date: November 15, 2011; Published Date: November 21, 2011
Citation: Baydack RS, McKenzie EJ, Robertson C, Booth SA, Jackson M, et al. (2011) Pre-Clinical Changes Observed by Magnetic Resonance Imaging in a Hamster Model of Transmissible Spongiform Encephalopathy: a Potential Biomarker of Prion Infection. J Mol Biomark Diagn 2:120. doi:10.4172/2155-9929.1000120
Copyright: © 2011 Baydack RS, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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The objective of the study was to develop a model for the diagnosis of prion diseases in live animals, using magnetic resonance imaging (MRI). Hamsters experimentally infected with the 263K strain of scrapie were imaged periodically during the course of prion infection. Changes in the brain, particularly the hippocampus, were observed during the first quarter of the incubation period. These changes included an increase in T2 relaxation time and apparent diffusion coefficient (ADC), indicative of an increase in the water content of tissues. These changes were apparent well before the appearance of clinical symptoms, and did not correlate with the typical histological changes characteristic of prion disease, (vacuolation, accumulation of PrP protein, gliosis) suggesting that the changes are caused by a progressive accumulation of fluid. This oedema may be a novel early marker of prion disease, and could play a role in pathogenesis.
Transmissible spongiform encephalopathies (TSEs), or prion diseases, are a group of invariably fatal neurodegenerative diseases of both humans and animals caused, in whole or in part, by an abnormallyfolded form of the normal cellular protein PrP .The normal form of this protein, known as PrPC, undergoes a conformational change during which it acquires a significant amount of β-sheet [2,3], producing the pathological form, PrPSc, which is partially protease resistant and insoluble [2,4].Prion diseases exist in genetic, sporadic, and acquired forms, all of which are transmissible . The most common human prion disease is Creutzfeld-Jakob Disease (CJD), the variant form (vCJD) of which is likely acquired through ingestion of beef from Bovine Spongiform Encephalopathy (BSE) infected cattle [1,6,7]. Other prion diseases include scrapie of sheep and goats, and Chronic Wasting Disease (CWD) of deer and elk in North America.
For research, rodent adapted prion strains, such as the 263K strain of scrapie in Syrian golden hamsters, are useful models due to the relatively short incubation period of 68-71 days following intracerebral infection [8-10]. Prion diseases are characterized by the deposition of PrPSc, the appearance of vacuoles, and gliosis in the brain . Different strains can be distinguished by the distributions of these histological markers in a given host. However, the unequivocal diagnosis of most prion diseases requires the post-mortem collection of central nervous system tissue, either for histological examination or Western blot analysis.
Since changes in soft tissues like the brain can be detected by magnetic resonance imaging (MRI), this method has been used to examine patients suspected of having CJD or other prion diseases. MR image abnormalities that have been reported include: increased signal on T2-weighted images in the basal ganglia [11,12],caudate nuclei and putamina , andcerebral cortex ; increased signal on T1- weighted images in the globus pallidus ; and increased signal on diffusion-weighted images in the cerebral cortex [15,16], basal ganglia , and striatum . In some cases, MR image abnormalities were compared to histological changes evident in tissues collected upon death. High signal intensity on diffusion-weighted [11,17], and T2- weighted  images correlated with a high degree of spongiform change. In experimentally-infected rodents, increased signal intensity on T2-weighted images correlated with gliosis [18,19] or PrPSc deposition ,while vacuolation correlated with decreased signal intensity .Only one previous report has described pre-clinical changes, and they were observed late in the pre-clinical stage of disease . We report here, for the first time, the observation by MRI of changes that occur very early following the infection of hamsters with the 263K strain of scrapie, during the first quarter of the incubation period. These changes involve increases in T2 relaxation time and apparent diffusion coefficient (ADC), and were evident well before the appearance of either clinical symptoms or the typical histological changes characteristic of prion disease. We conclude that the early MR abnormalities observed are the result of the progressive accumulation of fluid in the hippocampus, and that observation of these changes may be useful as a novel early biomarker of prion disease pathogenesis.
Magnetic Resonance Imaging - T2 Changes in Scrapie- Infected Hamsters
Syrian golden hamsters were inoculated intra-cerebrally with 263K scrapie brain homogenates. Magnetic resonance images were collected at 19; 33; 47; and 61 days post injection (dpi). At each time point a series of nine coronal images were collected (Figure 1). Differences were observed between infected hamsters and mock-infected or uninfected controls in slices five and six as early as the first time point (19 dpi).
Increases in T2 relaxation time were observed in the hippocampus of infected animals in MRI slices five and six (Figure 2 and Figure 3, respectively). The pixel intensity on T2-maps reflects the T2 relaxation time, which varies with specific experimental conditions (e.g. temperature). In order to compare images collected at different times, it was necessary to express the T2 relaxation time in the region of interest (ROI) in relative terms, and to define the ROI according to the boundaries of the damage observed on the images. Contour plots were used to define the ROIs in individual animals, by identifying areas where T2 relaxation time changed rapidly. In control and mockinfected hamsters, where contour plots did not identify affected regions, standard ROIs of a smaller size were used for comparison. Since minimal differences were observed in the thalamus between groups of hamsters at any time point, the T2 relaxation time in that region of the brain was used as a correction factor to account for differences in experimental conditions during the collection of MR images: the mean T2 relaxation time in the ROI was expressed as a percentage of that in the thalamus. Actual T2 relaxation times in affected regions at 61 dpi were on the order of 70 ms in slice 5 and 90 ms in slice 6; in the thalamus, T2 relaxation times measured in the low- to mid-fifties.
Figure 2: Increased T2 relaxation time in the hippocampus of infected hamsters in MRI slice 5. Top, false colour T2 maps of infected (left) and uninfected (right) hamsters collected at 61 days post injection (dpi). Bottom, mean relative T2 relaxation time in the hippocampus expressed as a percentage of the mean T2 relaxation time in the thalamus, +/- standard error of the mean (s.e.m.). Solid black line: infected; broken black line: mock-infected; solid grey line: control hamsters.
Figure 3: Increased T2 relaxation time in the hippocampus of infected hamsters in MRI slice 6. Top, false colour T2-weighted MR images of infected (left) and uninfected (right) hamsters collected at 61 days post injection (dpi). Bottom, mean T2 relaxation time in the hippocampus expressed as a percentage of the mean T2 relaxation time in the thalamus, +/- standard error of the mean (s.e.m.). Solid black line: infected; broken black line: mock-infected; solid grey line: control hamsters.
At 19 days post injection (dpi), scrapie-infected hamsters had a significantly different T2 value compared to mock-infected hamsters injected with PBS, and uninfected control hamsters (Figure 2). The difference between the infected and control groups was significant (p=0.0265, ANOVA). At 33 dpi, the T2 values of infected hamsters were significantly higher than those of mock-infected hamsters (p=0.0004).
The differences between infected and un-infected hamsters were more pronounced in MRI slice six at 47 and 61 dpi (Figure 3).At these time points, infected hamsters had significantly higher T2 values than both mock-infected and control hamsters, while there were no significant differences between the latter two groups. At 33 dpi, there were significant differences between scrapie-infected hamsters and un-injected controls. Taking MRI slices five and six into consideration together, differences between infected hamsters and both uninfected controls and mock-infected hamsters were statistically significant at 33, 47, and 61 dpi; at 19 dpi, infected hamsters could be distinguished from controls only.
Histological changes in scrapie-infected hamsters
In order to explore the causes of scrapie-induced changes on MR images of hamster brains, different brain regions were examined at each time point for three histological hallmarks of prion disease: spongiform change; accumulation of PrPSc; and gliosis (Figure 4). The hippocampus was chosen, along with three regions where no obvious differences were observed between infected, mock-infected, and control hamsters: the cortex at the level of the thalamus; the thalamus; and the hypothalamus. All four of these regions are part of the lesion profile commonly used to evaluate spongiform change in rodents with prion disease. Qualitatively, the thalamus was the region most affected by histological changes, particularly spongiform change and PrPSc deposition. In order to make a more quantitative assessment, images of histological slides were evaluated using image processing software, allowing measurements of vacuolation, accumulation of PrPSc, and gliosis. Since mock-infected and control hamsters yielded similar results on MRI, they were assembled into a single “Uninfected” group for the purpose of histological examination.
Figure 4: Histological changes in the hippocampus (A), thalamus (B), cortex (C), and hypothalamus (D) of scrapie-infected (first and third columns) and uninfected (second and fourth columns) hamsters, 61 days post injection. Haematoxylin and eosin staining for vacuoles: first and fourth rows; immunohistochemistry for PrP (second and fifth rows), and GFAP (third and sixth rows). Size bar = 50 microns.
A comparison between infected and uninfected hamsters shows differences in all three histological hallmarks of prion disease in the four regions of interest at 61 dpi (Figure 5). Significant differences in spongiform change were observed between infected and uninfected hamsters at 61 dpi in the thalamus, including the percentage of tissue covered by vacuoles (p=0.0137) and the total numbers of vacuoles (p=0.0164). There were also significant differences in anti-GFAP staining in both the cortex and the thalamus.
By examining the onset and progressing patterns of histological hallmarks of prion disease, we hoped to explain the differences observed on MR images. Beginning with spongiform change (Figure 6A), three of the regions of interest present similar curves: the cortex; the hippocampus; and the hypothalamus. In each case, vacuolation remains low in the earlier time points with a spike at 61 dpi; the levels observed in the hypothalamus remain lower than the other two regions throughout. In comparison, vacuolation in the thalamus is similar to that in the hypothalamus at the first time point, and then increases through the final two time points, making the thalamus the most highly vacuolated region at both 47 and 61 dpi. The curves of all four regions effectively parallel each other after the onset of significant changes; this seems to indicate that once spongiform change begins to occur, it appears at the same pace, regardless of brain region. Spongiform change cannot, however, be evaluated solely on the basis of the fraction of a brain section that is covered by vacuoles; the number of vacuoles present is also important. In the four brain regions studied, the curves representing area covered by vacuoles were effectively mirrored by those representing the number of vacuoles in a given area of tissue (Figure 7). In each case the increase in vacuole area, once initiated, was more rapid than that in number of vacuoles. Thus the total vacuole area increases not only with the addition of new vacuoles, but with the expansion of those vacuoles that were already present as well.
Figure 6: Progressive histological changes in four brain regions of scrapieinfected hamsters. Solid grey line: cortex; broken black line: hippocampus; broken grey line: hypothalamus; solid black line: thalamus. Measurements represent the proportion of slide area covered by vacuoles (A), PrP deposition (B), and gliosis (C).
Figure 7: Relationship between total vacuole area and the number of vacuoles in the cortex (A); hippocampus (B); hypothalamus (C); and thalamus (D) of scrapieinfected hamsters. Scale: number of vacuoles/digital image (solid black line); 100 x percentage of image area covered by vacuoles (broken black line)
Although PrPSc deposition follows a similar pattern to that of spongiform change, it is not identical (Figure 6B). Once again, the cortex, hippocampus, and hypothalamus share almost identical curves, with no real increase over the first three time points before an increase at 61 dpi. In this case, the level of PrPSc in the cortex at 61 dpi is clearly higher than that in either the hippocampus or the hypothalamus. PrPSc levels in the thalamus of infected animals increased at the first time point, and continued to increase throughout the time course. This increase became exponential over the last time point (note the scale break in Figure 6B).
All four brain regions showed similar gliosis levels between 19 and 47 dpi, with an increase at 61 dpi. The cortex and thalamus showed a greater increase at the end of the time period studied, with the highest final gliosis measurements.
The thalamus showed the greatest changes in histology compared to the other regions studied. Moreover, the onset of the changes was earlier, and in a specific order (Figure 8). In the cortex, hippocampus, and hypothalamus, changes in histology were not seen until 61 dpi (Figure 8A-C).
Figure 8: Patterns of histological changes in the cortex (A); hippocampus (B); hypothalamus (C); and thalamus (D) of scrapie-infected hamsters. Scale: 100 x percentage of image area covered by PrP (solid grey line) or vacuoles (broken black line); percentage of image area covered by gliosis (solid black line); number of vacuoles/digital image (broken grey line).
In the thalamus (Figure 8D) an increase in PrPSc levels was evident as early as 33 dpi; increased vacuole levels were apparent at 47 dpi; while proliferation of glial cells appeared at 61 dpi.
Apparent diffusion coefficient magnetic resonance imaging
Since it was apparent that abnormalities observed on T2 MR images appeared before histological changes, apparent diffusion coefficient (ADC) MR images were compared to T2-maps (Figure 9). It was determined that regions with increased T2 relaxation time (hyperintensities in the hippocampus in slices 5 and 6) corresponded to increased ADC values, indicating that image abnormalities may be the result of fluid accumulation in the hippocampus. In infected hamsters at 61 dpi, ADC values in the hippocampus were greatest in slice 5 (1.21 x 10-3 mm2/s) and slice 6 (1.71 x 10-3 mm2/s). In control and mockinfected hamsters, the highest single ADC value in the hippocampus at 61 dpi was 8.85 x 10-1 mm2/s in slice 5 and 1.20 x 10-3 mm2/s in slice 6.
Figure 9: Comparison of T2 maps and apparent diffusion coefficient (ADC) maps in the same scrapie-infected hamster between 19 and 61 dpi. First column: slice 5 T2 maps; second column: slice 5 ADC maps; third column: slice 6 T2 maps; fourth column: slice 6 ADC maps. Rows 1-4: images collected at 19, 33, 47, and 61 dpi, respectively.
In this study we were able to distinguish scrapie-infected hamsters from mock-infected and uninfected controls using MRI during the pre-clinical stage of disease.We also demonstrated that it is possible to follow disease progression longitudinally in individual animals, by imaging animals on successive time points. Furthermore, the differences observed in pre-clinical MR images did not correlate with the typical histological hallmarks of prion disease, and occurred earlier in the incubation period, potentially identifying a new marker for brain pathology. The fluid content of brain tissue appeared to increase in the hippocampus early in disease progression, as evidenced by changes in T2 relaxation time and apparent diffusion coefficient observed by MRI.
Intra-cerebrally inoculated hamsters were differentiated from mock-infected and uninfected controls by statistically significant hyperintensities in the hippocampus at 33, 47, and 61 dpi. All four of these time points were in the pre-clinical stage of disease; the documented incubation period of 263K scrapie in Syrian golden hamsters following i.c. infection is on the order of 68-71 dpi [8,9]. Although the 263K strain of scrapie is very fast acting, it was remarkable to find significant differences as early as we did. Abnormalities on MR images have previously been reported for prion diseases in both humans [12,14,20-22] and rodents [18,19,23], with variable results. In the case of the human studies, all MRI scans were carried out during the symptomatic stage of disease. To our knowledge, only one rodent study has identified pre-clinical differences between infected and control animals, at 120 dpi using the 139A strain of scrapie in mice . While it is difficult to compare disease stages in two different TSE strains and two different hosts, it seems apparent that the differences presented in the current study represent an earlier stage of disease: we observed changes at the one-quarter to one-third mark of the incubation period, compared to approximately the two-third mark in the previous study.
Our histological observations cannot explain the hyper-intensities found on T2-weighted images, since those MR abnormalities precede histological changes, and since the regions most affected according to MRI and histology are not the same.
The changes observed by MRI in our study could not have been due to vacuolation or gliosis, since the MRI changes occurred at time points well before the onset of significant spongiform changes and gliosis in the regions investigated. In the infected hamsters, there was significant spongiform change only at at 61 dpi, and in PrPSc deposition at 47 and 61 dpi in the thalamus; and in gliosis at 19 dpi in the hippocampus and at 61 dpi in both the cortex and the thalamus, as compared to uninfected controls. Thus the hippocampus, the region most affected according to MRI, did not have significant spongiform change over the course of the experiment, and only had slightly increased gliosis by the last time point. (61 dpi: Figure 6C).
The appearance of spongiform change, PrPSc deposition, and gliosis followed a common pattern in the hippocampus, cortex, and hypothalamus of 263K scrapie-infected hamsters: these results agree with previous studies involving the same scrapie and host strains and route of inoculation. Previous studies sequentially evaluated spongiform change and gliosis following i.c. infection in regions of the brain including those examined here [24,25]. The earliest observation of both pathologies occurred between 49 and 57 dpi , although only minor changes were observed at 52 dpi in the other study . Masters et al report that from 49-57 dpi onward, the degree of spongiform change in the cortex and the hippocampus is greater than in the thalamus and the hypothalamus, while gliosis is more prevalent in the thalamus and the cortex . These results support our findings. Two previous studies evaluating PrPSc distribution in the brains of infected hamsters between 7 and 65 dpi also support our results [26,27]. It was shown that PrPSc accumulated earliest and most significantly in the thalamus, beginning at 28-35 dpi, and that significant deposition in the cortex, hypothalamus, and hippocampus occurred only at 65 dpi
In a study carried out with μMRI on mice infected with scrapie, clinical symptoms appeared (around 150 dpi), and severe neurological deficits were present by 180 dpi . Hyper-intensities were observed in the septum, hippocampus, and cortex of infected mice, with corresponding increases in PrPSc deposition and gliosis, while spongiform change was not significant in these areas. A previous study using MR imaging on 263K scrapie-infected hamsters during the clinical stage of disease (74 dpi), compared the results to histological examination: neither spongiform change, gliosis nor PrPSc deposition correlated with MR changes .
T2 relaxation time is determined by the relative mobility of water molecules. Biofluids are characterized by relatively long T2 relaxation times, because of the high velocity of water molecules; dense, viscous fluids usually result in short T2 relaxation times due to the reduced velocity of water molecules. We believe that the observed increase in T2 relaxation time in the hippocampus of infected hamsters indicates an increase in fluid content. The correlation of increased ADC values with high T2 relaxation times supports this conclusion. The apparent diffusion coefficient is a measure of the mobility of water molecules. High ADC values correspond to free movement of water, while low values indicate that water movement is restricted, for example by cell membranes. Therefore, long T2 relaxation times and high ADC values observed in the hippocampus of infected hamsters would seem to indicate the accumulation of extracellular fluid, also known as vasogenic edema.
Cytotoxic edema is the result of accumulation of fluid inside cells, and is characterized by cellular swelling. Following initiation of swelling, individual oligodendrocytes  and neurons  mostly succumb to necrosis within 24 hours, while astrocytes begin to undergo necrosis after 24 hours . Therefore, if cytotoxic edema occurred, it would have only a transient effect on ADC values, but it would result in swelling followed by necrosis of neurons as well as glial cells. While hypertrophy of glial cells has been documented in this disease model , a high degree of gliosis only occurs at about 80 dpi . Because of this, we believe that the corresponding hyper-intensities on T2- and diffusion-weighted MR images are likely the result of vasogenic edema in the hippocampus of 263K scrapie-infected hamsters. In further support of this, breakdown of the blood-brain barrier, which is the cause of vasogenic edema , has been reported in experimental prion disease [23,33], in both cases evaluated by MRI. Chung et al reported disruption of the blood-brain barrier during clinical scrapie in hamsters , while Brandner et al observed progressive break down specific to neural grafts over-expressing PrPC in PrP null mice .
Increased expression of the water channels aquaporin-1 and -4 (AQP1 and AQP4) has been reported in cases of CJD, as well as in BSE-infected bovine-PrP transgenic mice . In the case of the transgenic mice, AQP1 and AQP4 levels were elevated only at 270 dpi, when vacuoles and PrPSc deposits appeared. Expression of AQP4, the main water channel in the brain, is thought to be regulated through protein kinase C (PKC) and/or mitogen-activated protein kinase (MAPK) signaling pathways. Specifically, AQP4 mRNA and protein levels are increased through a p38 MAPK-dependent pathway , and inhibited in a PKC-dependent manner . In addition, PKC has been shown to inhibit the activity of AQP4 by phosphorylating it, causing a decrease in its permeability to water [37,38]. PrPC has been implicated in cell signaling, including through both MAPK- [39,40] and PKC-dependent pathways . The sequential activation of p38 MAPK has been demonstrated from 50 dpi onward in 263K scrapieinfected hamsters . It is tempting to speculate that dysregulation of p38 MAPK and/or PKC signaling pathways due to a loss/modulation of normal PrPC function may be responsible for the increased expression of AQP4 observed during the clinical stage of prion disease.
As a water channel, AQP4 is important in maintaining water homeostasis in the brain, and is therefore implicated in cerebral edema. AQP4 null mice have been evaluated in their response to both vasogenic and cytotoxic edema. The absence of AQP4 was associated with improved neurological outcome and reduced brain swelling in models of cytotoxic edema including water intoxication and ischemic stroke , and pneumococcal meningitis . In contrast, AQP4 null mice faired worse than controls in models of vasogenic edema including staphylococcal brain abscess , and intraparenchymal fluid infusion, freeze-injury, and brain tumour . These observations indicate that AQP4 is helpful in combating vasogenic cerebral edema, but detrimental in cases of cytotoxic edema. PrPC expression is upregulated following focal cerebral ischemia , a model of cytotoxic edema, and PrPC deletion leads to worsened outcome following ischemic brain injury [48,49]. This supports the theory that loss of PrPC function late in prion disease pathogenesis results in increased AQP4 expression, since increased AQP4 expression would be detrimental following ischemic brain injury. We believe that the chronic edema reported in this study is vasogenic in nature .
We have demonstrated for the first time progressive increases in both T2 relaxation time and apparent diffusion coefficient in the hippocampus of scrapie-infected hamsters beginning very early in disease progression. These MR image abnormalities are indicative of accumulation of fluid in the hippocampus, occurring earlier than any prion disease-associated histological changes. We speculate that alterations in AQP4 expression previously reported during clinical disease  may result in initiation of edema, which may play a part in glial cell swelling and neuronal death. Confirmation of the exact role of edema in prion disease must await further studies.
Five-week-old Syrian golden hamsters were injected intracerebrally with 50 microlitres of 1% (w/v) 263 K scrapie brain homogenate in phosphate buffered saline (PBS), obtained from the TSE Resource Centre, Institute for Animal Health (Compton, Newbury, Berkshire, UK) as a stock 10% suspension. Mock-infected hamsters were injected with 50 microlitres of PBS only, and controls received no injection. Injections were performed into the right parietal region of the brain under isoflurane anaesthetic, using a needle fitted with a guard to prevent it from penetrating too deeply.
Magnetic Resonance Imaging
Beginning at 19 days post injection (dpi), hamsters underwent magnetic resonance imaging (MRI) every two weeks until either 47 or 61 dpi. Imaging was performed using a Bruker 7 Tesla scanner. Hamsters were enclosed in a custom-made holder, and breathing and temperature were monitored throughout imaging under isoflurane anaesthetic. A custom-made quadrature coil was used, and T2-weighted and diffusion weighted images were acquired using respiratory gating as a trigger for the Radio Frequency (RF) sequence. Nine coronal slices were collected, 1.7 mm thick and 2 mm apart. T2 parameters: TR=1450 ms; TE=15 ms; 6 echoes (at multiple times of TE); field of view 3 x 3 cm; data matrix 256 x 128; 1 average. Diffusion-weighted imaging parameters (spin-echo sequence): TR=1200 ms; TE=40 ms; 1 echo; field of view 3 x 3 cm; data matrix 256 x 64. T2-maps were produced from T2-weighted data; apparent diffusion coefficient (ADC) maps were produced from diffusion-weighted data.
Collection of Tissues
Hamsters having undergone MRI were euthanized at 48 or 62 dpi by intra-cardiac injection of Euthanol. Each hamster brain was removed and frozen at -80°C for infrared microspectroscopic analysis, and for production of brain homogenate for subsequent infections. Hamsters to be examined histologically were euthanized in the same manner at 19, 33, 47, or 61 dpi. Upon removal, brains were fixed in formalin, dehydrated, and embedded in paraffin for sectioning.
Paraffin-embedded sections 5-6 microns thick were examined for the histological hallmarks of prion disease. Sections were stained with hematoxylin and eosin for evaluation of spongiform change using standard procedures. Other sections were probed with anti-PrP or anti-GFAP (glial fibrillary acidic protein) antibodies according to the manufacturer’s directions to evaluate PrPSc deposition and gliosis, respectively. Before immunohistochemistry for PrP, epitopes were exposed on sections by autoclaving with HCl. Monoclonal antibody 3F4 was used to identify PrP deposits, with visualization using a biotinylated goat anti-mouse secondary antibody and DAB with horseradish peroxidase. Polyclonal anti-GFAP antibody raised in rabbit was used to stain glial cells, using the same visualization method, substituting a goat anti-rabbit secondary antibody.
Quantification of Histological changes
Images of stained sections were acquired using a Leica light microscope fitted with a digital camera. ImagePro software was used to quantify spongiform change, PrPSc deposition and glial cell staining in four selected regions of the brain: the hippocampus; the retrosplenial cortex; the thalamus; and the hypothalamus. For images of sections stained immunohistochemically, the software was used to create a template of the colour range identifiable as being immuno-stained. This template was then applied to all images, and a measurement, consisting of the percentage of the total image area that was covered by immunostaining, was produced. In the case of gliosis, the measurement was expressed as the raw percentage, whereas PrPSc deposition was expressed as 100 x % area covered.
In order to measure spongiform change, ImagePro software was used to evaluate potential vacuoles based on their size and shape. The size and shape restrictions were based on those set out by Sutherland et al. [51,52], except that vacuoles of a smaller size were included in this analysis (minimum of 8 square microns compared to 69). Potential vacuoles whose aspect ratios exceeded 3 or whose convex hull area to object area ratios exceeded 1.7 were eliminated. Before any measurements were collected or potential vacuoles were excluded, a series of image processing steps was applied to precisely define the boundaries of each object. The image was converted to greyscale and a Fast Fourier Transform was performed. A low-pass filter was then applied using Hanning filtering to attenuate high frequency information in the image, and an inverse Fourier Transform was executed. Finally, an intensity threshold was applied, eliminating lower intensity areas of the image, and leaving only potential vacuoles.
MR images were processed using in-house developed software (Marevisi). Contour plots of T2 relaxation time were created from the multi-echo data sets in order to identify regions that were consistently affected in infected hamsters.
In order to compare the mean T2 relaxation time in the region identified (the hippocampus in two consecutive brain slices), in images collected at different times, this measurement was expressed as a percentage of the mean T2 relaxation time in the thalamus of the same image. This region was chosen because minimal differences were observed in the thalamus between hamster groups at any of the four time points studied. In this way, the measurements being compared were corrected for differences in experimental conditions during MR image collection. At each of the four time points, an ANOVA was carried out to compare the corrected T2 values in the hippocampus in the three hamster groups. In certain instances, comparison by ANOVA was not possible due to heterogeneity of variances between groups. In these cases, groups were compared using the Kruskall-Wallis test. ADC images were processed in the same manner.
For the purpose of comparison of histological hallmarks of prion disease, mock-infected and control hamsters were grouped together as a single group, called Uninfected, since MRI results were similar for both groups, and since neither were expected to show significant spongiform change or PrPSc deposition. At each time point, and for each histological characteristic being studied, two analyses were conducted: a series of t-tests comparing infected and uninfected groups in each of the four regions of interest; and an one way ANOVA comparing the four regions of interest in infected animals only.
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