|"Elevated pressure extraction experiments were carried out using a home built pressure instrument. Samples were heated at 65â100Â°C under a constant pressure of 45,000 psi in stainless steel reaction vessel coupled to a manually operated HiP High Pressure Generator (High Pressure Equipment Company, Erie, PA, USA). This instrument uses a hydraulic screw pump to compress fluid in order to generate pressure. Since water is virtually non-compressible, in the event of a system failure, discharge forces are minimal and do not present a hazard to equipment or personnel.
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Citation: Fowler CB, OâLearyTJ, MasonJT (2014) Improving the ProteomicAnalysis of Archival Tissue by Using Pressure-Assisted Protein Extraction:A Mechanistic Approach.J Proteomics Bioinform7: 151-157."