alexa A Comparative Study Of Thermo-inducible HSP70 Gene Expression Pattern In Clinical And Environmental Isolates Of Aspergillus Fumigatus In A Time-course Manner | 60951
ISSN: 2157-7579

Journal of Veterinary Science & Technology
Open Access

Like us on:
OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

6th Global Veterinary Summit
November 14-16, 2016 Atlanta, USA

Golnaz Sharafi, Ramak Yahya Rayat, Ghasem Vahedi and Alireza Khosravi
University of Tehran, Iran
ScientificTracks Abstracts: J Vet Sci Technol
DOI: 10.4172/2157-7579.C1.021
Abstract
Introduction & Aim: Aspergillus fumigatus is the most frequent etiologic agent of invasive Aspergillosis in immunocompromised patients. Economic significance of Aspergillus spp., in animal health is mainly characterized by their ability in production of mycotoxins and cause disease in respiratory system of poultry. The ability of a pathogen to adapt to a high temperature environment such as that in the host body is the key factor for the pathogenesis. HSP70 family of genes and their related proteins play a pivotal role in resistance of microbial cells to stressed conditions within host body. Here, we examined relative gene expression of heat-inducible HSP70 gene in environmental and clinical (from infected ostriches) isolates of Aspergillus fumigatus. Methods: The isolates were cultured at four temperature-points (20°C, 30°C, 37°C and 42°C) for five days, quantitative realtime PCR used to measure the relative gene expression of HSP70 gene in clinical isolates in comparison to Aspergillus fumigatus ATCC 90906 standard strain which was incubated at 25 °C for five days. Results: During entire five days HSP70 expression level in clinical samples was higher than in environmental samples (p<0.05, Mann Whitney U test). Difference in expression level between two groups at 42 °C was reduced. Mean HSP70 expression level of five incubation days showed a slow and constantly increasing pattern by temperature elevation in both groups at 30 °C, 37 °C and 42 °C but in contrary at 20 °C both groups demonstrated a decreasing expression pattern. Temperature shift from 20 °C to 42 °C resulted in a strong HSP70 induction and up to 10 and 8.6 fold change in its expression levels at the end of fifth day of incubation in clinical and environmental groups, respectively. Conclusion: We concluded that two temperature-points including 37 °C and 42 °C are major temperature-points inducing HSP70 expression in Aspergillus fumigatus and cause highest expression shift in both experimental groups.
Biography

Golnaz Sharafi has completed her DVM with excellent grade from Veterinary School in Tehran University, Iran. She is currently a R&D and Lab Officer in Viromed Laboratory. She has published more than 5 papers in reputed journals and also attended several international conferences.

Email: [email protected]

image PDF   |   image HTML
 
Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2018-19
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri & Aquaculture Journals

Dr. Krish

[email protected]

1-702-714-7001Extn: 9040

Biochemistry Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals

Ronald

[email protected]

1-702-714-7001Extn: 9042

Chemistry Journals

Gabriel Shaw

[email protected]

1-702-714-7001Extn: 9040

Clinical Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Engineering Journals

James Franklin

[email protected]

1-702-714-7001Extn: 9042

Food & Nutrition Journals

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

General Science

Andrea Jason

[email protected]

1-702-714-7001Extn: 9043

Genetics & Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Materials Science Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Nursing & Health Care Journals

Stephanie Skinner

[email protected]omicsonline.com

1-702-714-7001Extn: 9039

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001Extn: 9038

Neuroscience & Psychology Journals

Nathan T

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

Ann Jose

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001Extn: 9042

 
© 2008- 2018 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version