Journal of Bacteriology & Parasitology

A loop- mediated amplification (LAMP)-based detection method for Salmonella in animal feed

International Congress on Bacteriology & Infectious Diseases

November 20-22, 2013 DoubleTree by Hilton Baltimore-BWI Airport, MD, USA

Martin D'Agostino

Accepted Abstracts: J Bacteriol Parasitol

Abstract:

Loop-mediated amplification (LAMP) is an alternative nucleic acid amplification to the polymerase chain reaction (PCR). It uses 6 oligonucleotide primers to mediate amplification of target sequences, which confers high specificity on the assay. LAMP operates with great rapidity, and amplification products can be detected within a few minutes of the start of the reaction. Unlike PCR, LAMP operates isothermally, and thus does not require thermocycling equipment. LAMP instrumentation is highly portable, and thus has the potential to be used outside the analytical laboratory. In the analysis of feedstuff materials for the presence of pathogenic microorganisms, this could be an advantageous feature, potentially facilitating the screening of samples at-site to allow a rapid turnaround of materials. A LAMP-based method has been developed for the detection of Salmonella in animal feed stuffs. The method is based on the assay of Zhang et al. 2011 (Appl. Env. Microbiol. 77 6495-6501), modified to contain controls to identify correctly performed reactions. The assay is harnessed to a sample treatment comprising standard culturing procedures, to be fully compatible with current practice. The method should be useful for analysis of a wide range of feedstuff materials for the presence of Salmonella species.

Biography :

Martin D?Agostino has worked for the Food and Environment Research Agency (FERA) since 1994. His microbiological career began with Kennerty Farm Dairies in Aberdeen (now Robert Wisemans) as a laboratory assistant, until he joined what was the Torry Food Science Laboratory (Central Science Laboratory, MAFF) in 1994, now named the Food and Environment Research Agency as part of Defra. He has been involved in EU Framework 5, 6 and 7 projects dealing with such areas as Food PCR, Surveillance of Adenovirus and Norovirus in European Bathing waters and more recently the FP7 project entitled ?Integrated monitoring and Control of Foodborne Viruses in European Food Supply Chains?. This has resulted in the accumulation of many European and worldwide contacts from both Academia and Industry related to both food and environmental microbiology. He has eexperience of chairing and presenting scientific talks at international scientific meetings and has been author in at least 17 papers, several reviews and book chapters related to the detection and implementation of rapid methods for foodborne and environmental human pathogens. He is a member of the Standing Committee of Analysts (Environment Agency) and has been an active participant in the CEN/TC 275/ WG 6/TAG 3 ? ?PCR for the Detection of Food-borne Pathogens? standardisation body. He regularly presents and chairs at international conferences and working groups on EU projects and has published several papers on environmental virology and food microbiology. ?H? Index is currently 10.