Journal of Chromatography & Separation Techniques
Open Access
ISSN: 2157-7064
+44 1300 500008
ISSN: 2157-7064
+44 1300 500008
Ouassila Ferroukhi, Nihad Mermat, Saliha Guermouche, Jean-Pierre Bayle and Moulay Hassene Guermouche
University of Science and Technology Houari Boumediene, Algeria
University of Paris-Sud, France
Posters & Accepted Abstracts: J Chromatogr Sep Tech
The development of new bonded stationary phases is an important aspect in reversed-phase liquid chromatography (RPLC) the most used mode in high-performance LC. A novel mesogenic ether crown stationary phase has been synthesized and characterized (figure 1). The phase was obtained by coupling between Lichrospher Si 100 NH2 and the mesogenic carboxylic ether crown acid liquid crystal ECLC. Characterization of ECLC was made with proton NMR, and the nematic state was determined by DSC. Thermal study of the new material exhibit transitions in Vanâ�?�?t Hoff plots indicating changes of the structure of the phase during heating. Analytical chromatographic behaviors of the new bonded liquid crystal stationary phase BLCSP were investigated by reversed phase LC. Separation of poly-aromatic hydrocarbons (PAHs) is described using high water content mobile phase. Bonded materials exhibit a liquid crystal-like behavior and molecular shape recognition toward planar and non-planar solutes probably due to the mesogenic state. The long nematic chain combined with a terminal ether crown imparted the new stationary phase fine selectivity towards PAH isomers. The shape selectivity demonstrated by the BLCP is higher before the transition occurring around 40�?°C. Using acetonitrile/ water (35/65), reversed phase data of polyaromatic hydrocarbons showed significant solute planarity recognition of anthracene/oterphenyl. The shape discrimination was evaluated by some pairs of isomers like phenanthrene/anthracene and chrysene/tetracene. The linear solute with the high length to bread ratio L/B is more retained on the new stationary phase.
Email: ferroukhiouassila@yahoo.fr