alexa A Novel Real-time PCR Quantitative Method Of Crab Species
ISSN: 2155-9600

Journal of Nutrition & Food Sciences
Open Access

Like us on:
OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Share This Page

Additional Info

Loading
Loading Please wait..
 

10th World Congress on Nutrition & Food Sciences
May 29-31, 2017 Osaka, Japan

Zi-Yu Song and Cheng-Hong Hsieh
Asia University, Taiwan
Posters & Accepted Abstracts: J Nutr Food Sci
DOI: 10.4172/2155-9600-C1-045
Abstract
Meat adulteration has posed considerable risks to public health, and quantitative methody real-time PCR (qPCR) is a powerful tool used for both research and diagnostic, which has the advantages, compared to relative quantification, of providing an absolute copy number for a particular target. However, reliable standard is important for qPCR by using genome synthesis of generic crab fragments as a standard (fragments region is 16s rRNA) . In this study, we developed a novel real-time PCR quantitative method for the detection of crab species that are used as meat products or meat adulterants, And design generic primers and probes for crabs, and then calculate copy number of crabs. The results confirmed crab species could be tested to calculate their copy number. The limit of quantification was 12 copies of crabs genomic DNA or 1% meat ingredient. This assay enables detection of multiple species of crab copy number. This is also applicable to other adulterated foods and genetically modified foods.
Biography

Zi-Yu Song graduated from the Asia University of Food Nutrition and Biotechnology, after graduation continue to study, studying at the Asian University Master student. Because Taiwan is surrounded by the sea, the sea food is very rich in resources, many people poisoning in Taiwan because of eating toxic crab. So my direction of the study of the crab qualitative, quantitative, and calculate the copies, the future will continue to study crab processed products, and combined with the detection of toxic crabs(TTX), the future hope that I can make effort to protect the Taiwanese food safety issues.

Email: [email protected]

image PDF   |   image HTML
 
Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri, Food, Aqua and Veterinary Science Journals

Dr. Krish

[email protected]

1-702-714-7001 Extn: 9040

Clinical and Biochemistry Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals

Ronald

[email protected]

1-702-714-7001Extn: 9042

Chemical Engineering and Chemistry Journals

Gabriel Shaw

[email protected]

1-702-714-7001 Extn: 9040

Earth & Environmental Sciences

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

Engineering Journals

James Franklin

[email protected]

1-702-714-7001Extn: 9042

General Science and Health care Journals

Andrea Jason

[email protected]

1-702-714-7001Extn: 9043

Genetics and Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001 Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Informatics Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Material Sciences Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Mathematics and Physics Journals

Jim Willison

[email protected]

1-702-714-7001 Extn: 9042

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001 Extn: 9038

Neuroscience & Psychology Journals

Nathan T

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

John Behannon

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001 Extn: 9042

 
© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
adwords