Reach Us +1-217-403-9671
A Point Mutation At The Vicinity Of Active Site Of A Lipase Enhances The Protein Thermostability Of A Mutant Several Fold | 9069
ISSN: 0974-276X

Journal of Proteomics & Bioinformatics
Open Access

Like us on:

OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations
700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

A point mutation at the vicinity of active site of a lipase enhances the protein thermostability of a mutant several fold

3rd International Conference on Proteomics & Bioinformatics

Pushpender Kumar Sharma, and Jagdeep Kaur

AcceptedAbstracts: J Proteomics Bioinform

DOI: 10.4172/0974-276X.S1.069

In the present investigation, a thermostable mutant lipase was generated employing directed evolution approach. The mutant exhibited 144 fold enhanced thermostability over the wild type enzyme at 60?C. The mutant enzyme showed high catalytic efficiency (kcat/Km). Biophysical investigation employing circular dichroism spectroscopy demonstrated that the mutant enzyme retained its secondary structure up to 70?80?C, whereas the Wt type protein structure was completely distorted above 35?C. In addition to this, the intrinsic tryptophan fluorescence conducted for the Wt and mutant enzyme also displayed variation in conformational changes during temperature dependent unfolding. By comparing the modelled structure of the Wt and mutant enzyme we found that the mutant N355K mutation exhibited extensive H-bonding (Lys355 HZ1\OE2 Glu284) with a distance 2.44 ?, whereas the Wt enzyme has not shown such H bonding interaction.
Pushpender K. Sharma, presently working as Assistant Professor at Sri Guru Granth Sahib World University, Fatehgarah sahib, Punjab, India. He completed is Postdoctoral fellowship in department of biotechnology at IISER, Mohali, India.
Leave Your Message 24x7