This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)
All submissions of the EM system will be redirected to Online Manuscript Submission System. Authors are requested to submit articles directly to Online Manuscript Submission System of respective journal.
rolonged diabetes mellitus (DM) induced endothelial progenitor cell (EPC) dysfunction causes impaired wound healing
which may be rescued by delivery of large numbers of ?normal? EPCs to wound sites. The challenge herein isculturing the
number of EPCs required and in achieving sustained delivery onto wound sites for effective participation in the diabetic wound
healing process. Most known scaffolds allow adhesion, but not growth or proliferation of EPCs and may / may not permit delivery
onto wound sites. Clearly, a matrix that can perform a multitude of functions is the need of the day.
To remedy these lacunae, we standardized direct seeding and attachment of murine bone marrow MNCs onto a uniformly
smooth polycaprolactone : gelatine (PCG) nano-fibre matrix, followed by 14 day culture. Cellularsaays such as CAA, CFC,
proliferation and viability assays revealed that seeding of BM-MNCs directly onto the PCG matrix allowsenhanced 4.42 fold
attachment of BM- MNCs, andpromoted growth, colony formation (1.33 fold) as well as proliferation (1.25 fold)as compared to
controls, without compromising viability of EPCs. In vitro, the PCG-matrix allows chemotactic migration of cells while facilitating
sustained delivery of EPCs onto diabetic wounds, resulting in a pathologically significant, scar-freehealing of diabetic wounds.
Our data, thus, highlight the novel therapeutic potential of the ?PCG-embedded EPCs? - as a combined ?growth and delivery
system?- functioning both as a ?substrate? for direct seeding, and growth of EPCs and as a ?scaffold? for direct application onto
Meghana has completed her Ph.D in 2007 and is currently pursuing her postdoctoral research in the National Centre for Cell Science, Pune, India.
Her research interests are EPC dysfunction and rescue for improved wound healing in diabetes mellitus
Peer Reviewed Journals
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals