Oral Health and Dental Management

Adipose-derived mesenchymal stem cell tracking using green fluorescent protein

4^th Asia Pacific Congress & Expo on Dental and Oral Health

July 27-29, 2015 Brisbane, Australia

Zahra Sarabadani

Posters-Accepted Abstracts: Oral Health Dent Manag

Abstract:

In 2002, researchers at UCLA published a manuscript in Molecular Biology of the Cell describing a novel adult stem cell population isolated from adipose tissue?the adipose-derived stem cell (ASC). Since that time, the ASC has gone on to be one of the most popular adult stem cell populations currently being used in the stem cell field. Many different roles for stem cells exist in varying regeneration processes. The capacity of adipose-derived stem cells (ASCs) to promote angiogenesis, secrete growth factors, regulate the inflammatory process, and differentiate into multiple cell types makes them a potential ideal therapy for medicine applications. The aim of this study was to cell tracking of mesenchymal stem cell derived from intra scapular adipose tissue of rabbit with green fluorescent protein. In this study adipose tissue of intra scapular region of 12 male New Zealand rabbits was harvested during the direct surgery or liposuction. After derivation of stem cells they were tracking via green fluorescent protein of lentivirus. However, our study has confirmed the ability of getting stem cell from adipose tissue it seems that more investigation is necessary for the suitable conditions and characteristics of their production.