alexa Agrobacterium: A Suitable Genetic System For Analysis Resistance To Xenorhabdus Antimicrobial Peptide Complexes Extremely Active In Food Spoilage (zoonic) Bacteria
ISSN: 1948-593X

Journal of Bioanalysis & Biomedicine
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8th World congress on Bioavailability & Bioequivalence: Pharmaceutical R & D Summit
June 26-27, 2017 San Diego, USA

Andras Fodor
University of Pannonia, Keszthely, Hungary
ScientificTracks Abstracts: J Bioanal Biomed
DOI: 10.4172/1948-593X-C1-029
Abstract
Increasing challenge n prevention and control in microbial (zoonic) pathogens in foods. The aim of this work is to establish a suitable experimental system for genetic analysis of antimicrobial peptide-resistance in a genetically well-characterized Gram-negative target, and found Agrobacterium tumefaciens. Intentionally, not a single but a multiple antimicrobial peptidecomplex (EMA_PF2, isolated from Xenorhabdus budapestensis, with a reproducible MALDI profile), was chosen as a model to be bio-assayed in liquid cultures. Antimicrobial activity of EMA_PF2 is demonstrated on t Gram-positive Rhodococcus equi, Erysipelothrix rhusiopathiae, Staphylococcus aureus, Streptococcus equi, Corynebacterium pseudotuberculosis, and Listeria monocytogenes) and Gram negative Salmonella Gallinarum, Salmonella Derby, Bordetella bronchiseptica, Escherichia coli, Pasteurella multocida, Aeromonas hydrophila strains including multi-resistant ones. A. tumefaciens A281 strain with C58 chromosome (originated from a nopaline-catabolizing strain) and intact (transfer-DNA, T-DNA carrying) pTiBo542 plasmid (with agropine (L, L, -succinamopine) catabolizing genes) proved fully resistant to EMA_PF2; while the studied disarmed (transfer-DNA-deleted, del-T-DNA) plasmid-harboring derivatives (AGL1; EHA105 and A4T) were fully sensitive. By contrast, all 5 examined pTi58-plasmid-cured derivatives of C58 nopaline-catabolizing strains proved resistant to EMA_ PF2. Two octopine-catabolizing strains behaved differently. Agrobacterium strains sensitive to EMA_PF2, harboring disarmed pTiBo542 provide a system for genetic complementation analysis of resistance to antimicrobial peptides using complementary sequences from resistant strains.
Biography

Present position: Retired from Senior Research Associate position from the University of Pannonia, Georgikon Faculty, Department of the Animal Sciences & Animal Breeding, Diploma: Geneticist – biologist & Teacher Certification for High School, 234/1964 PhD: Committee for Doctoral Awards of the Hungarian Academy of Sciences, October 7, 1974, Budapest, Hungary; PhD: Candidate of Biological Sciences; (No. 6.162). Biological Doctor’s Degree: D-1674/1974, Eötvös Loránd University, Budapest, Hungary, December 3, 1974. Habilitation: Habilitation Committee of the Eötvös Loránd University, Budapest, Hungary, December 13, 2000, Budapest, Hungary. Széchenyi Professorship Award: March 19, 1999, Hungarian Ministry of Education Fulbright Research Grant, a Fulbright Grant Biological Science Grant (1214102) and funds from Valent Biosciences, both awarded to András Fodor to conduct research in the lab of Heidi Goodrich-Blair at the University of Wisconsin-Madison, USA, 2015.

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