alexa An Atypical Human Endogenous Retrovirus, ERV3 Env, Induces Human Chorionic Gonadotropin (β-hCG) In A Model Of Placental Trophoblast
ISSN: 1948-5964

Journal of Antivirals & Antiretrovirals
Open Access

Like us on:
OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

4th World Congress on Virology
October 06-08, 2014 Hilton San Antonio Airport, TX, USA

Neal S Rote
ScientificTracks Abstracts: J Antivir Antiretrovir
DOI: 10.4172/1948-5964.S1.020
Abstract
Normal human placentation requires differentiation of specialized cells (villous cyto-trophoblast into syncytiotrophoblast); principally characterized by intercellular fusion and production of the hormone chorionic gonadotropin (hCG). Concurrently trophoblasts ex-press cellular genes of apparent retroviral origin (endogenous retroviruses; ERV), includ-ing endogenous retroviral element ERV3 env. We reported that, unlike other retroviral env regions that encode fusion proteins, ERV3 env regulated induction of the β subunit of hCG (β-hCG). The biological relevance of ERV3 env was questioned in a report of adults with homozygous stop mutations leading to a ?natural knockout? of ERV3 env alt-hough a truncated (p25) SU protein was produced that lacked the biologically active regions typically attributed to exogenous or endogenous retroviral Env proteins. The p25 region was never tested for capacity to induce β-hCG. ERV3 env contains two proposed translational start sites at nt 595 and nt 715. We cloned and inserted the entire ERV3 env open reading frame (ERV3), the SU region, and the p25 region, beginning at both proposed start sites, in stable expression vectors into BeWo cells (a model for villous cytotrophoblast differentiation when treated with forskolin), quantified levels of intracellular β-hCG and actin by western blot analysis, and data expressed as means (SD) of the ratio of β-hCG to actin of three independent experiments. β-hCG was not detectable in untreated BeWo or those transfected with vector alone (negative controls) and maximally expressed in forskolin-treated cells (positive control; 1.83 + 0.83). Transfection with ERV3, ERV3 SU, and p25 induced significantly (P<0.01) greater levels of β-hCG expression. BeWo cells were also stably transfected with vectors expressing siRNAs targeted to regions near the start sites, and the cell lines treated with forskolin or vehicle alone for 24, 48, and 72 hr. Transfection with si670, targeted to nt 670- 688, between the proposed start sites, completely prevented induction of β-hCG by forskolin at all time points. Thus, ERV3 env is an atypical retroviral element with a unique trophoblast hormone regulatory site in the SU region and in the p25 truncated protein expressed in individuals with described homozygous stop mutations.
Biography
Neal S. Rote completed his Ph.D. at Temple University School of Medicine and postdoc-toral studies at Heidelberg University and UCLA School of Medicine. He is the William Weir, M.D. Professor of Reproductive Biology and Professor of Pathology at Case West-ern Reserve University School of Medicine and Academic Vice Chair and Director of Research in the Department of Obstetrics and Gynecology, University Hospitals Case Medical Center, Cleveland, OH. He has published more than 110 papers in reproductive biology and 75 chapters and books, been NIH-funded for 32 years, and served on many NIH review committees.
image PDF   |   image HTML
 
Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri & Aquaculture Journals

Dr. Krish

[email protected].com

1-702-714-7001Extn: 9040

Biochemistry Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals

Ronald

[email protected]

1-702-714-7001Extn: 9042

Chemistry Journals

Gabriel Shaw

[email protected]

1-702-714-7001Extn: 9040

Clinical Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Engineering Journals

James Franklin

[email protected]

1-702-714-7001Extn: 9042

Food & Nutrition Journals

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

General Science

Andrea Jason

[email protected]

1-702-714-7001Extn: 9043

Genetics & Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Materials Science Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Nursing & Health Care Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001Extn: 9038

Neuroscience & Psychology Journals

Nathan T

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

Ann Jose

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001Extn: 9042

 
© 2008- 2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version