alexa An LC-MS/MS Method For The Determination Of Cucurbitacin E In Rat Plasma For Pharmacokinetic Studies | 19825
ISSN: 0975-0851

Journal of Bioequivalence & Bioavailability
Open Access

Like us on:

OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations
700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

5th World Congress on Bioavailability and Bioequivalence Pharmaceutical R&D Summit

Giovana Maria Lanchoti Fiori
Accepted Abstracts: J Bioequiv Availab
DOI: 10.4172/0975-0851.S1.019
Abstract
C ucurbitacins are highly oxygenated tetracyclic triterpenes isolated mainly from Cucurbitaceae plant family. Cucurbitacin E is the most studied Cucurbitacin due to its anti-proliferative activity against various types of cancer. However, there are no experimental studies regarding to its pharmacokinetics in plasma samples. The objective of the present study was to develop and validate a LC-MS/MS method for the quantitative analysis of Cucurbitacin E in rat plasma with application in pharmacokinetic studies. Wistar male rats received a single oral or a single intraperitonial 1mg/kg dose of Cucurbitacin E in saline. Serial blood samples were collected from 0-24 h after drug administration. Aliquots of 100 μL plasma were extracted with acetonitrile:methyl tert-butylether (1:5) and added with clobazam as internal standard. The samples were injected on a Select B column using a mixture of methanol:acetonitrile:water (30:40:30) as a mobile phase. Analyses were performed by LC-MS/MS consisted of an ESI interface operating at positive ionization mode and multiple reaction monitoring. Method validation included matrix effect, linearity, LOQ, stability, within-run and between-run precision and accuracy. The method showed linearity in the range 0.04-10 ng/mL with a LOQ of 40 pg/mL. The method applied in the evaluation of Cucurbitacin E pharmacokinetics administered to rats orally or i.p. showed that Cucurbitacin E was not detected in concentrations ≥ 40 pg/mL (LOQ) in all collected plasma samples. In conclusion, the pharmacokinetics of Cucurbitacin E in plasma should be evaluated using its metabolites, such as Cucurbitacin I identified in rat urine in a previous study of the research group.
image PDF   |   image HTML
 

Relevant Topics

Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2018-19
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri & Aquaculture Journals

Dr. Krish

[email protected]

1-702-714-7001Extn: 9040

Biochemistry Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals

Ronald

[email protected]

1-702-714-7001Extn: 9042

Chemistry Journals

Gabriel Shaw

[email protected]

1-702-714-7001Extn: 9040

Clinical Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Engineering Journals

James Franklin

[email protected]

1-702-714-7001Extn: 9042

Food & Nutrition Journals

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

General Science

Andrea Jason

[email protected]

1-702-714-7001Extn: 9043

Genetics & Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Materials Science Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Nursing & Health Care Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001Extn: 9038

Neuroscience & Psychology Journals

Nathan T

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

Ann Jose

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001Extn: 9042

 
© 2008- 2018 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
Leave Your Message 24x7