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|Susanne Rauch, Lutz J, Lazzaro S, Schmidt K, Petsch B, Baumhof P, Heidenreich H and Fotin-Mleczek M|
|ScientificTracks Abstracts: J Vaccines Vaccin|
|In recent years, messenger RNA (mRNA) based technologies have increasingly been applied in vaccine development. Such approaches have utilized mRNA for both therapeutic cancer vaccinations, and for prophylactic vaccines, drawing much attention from industrial and academic fields. RNActive®, an mRNA based vaccination technology, has yielded promising results in the development of vaccines against a variety of viral pathogens such as RSV (respiratory syncytial virus), influenza, rabies, and Ebola virus in several animal models. We have previously shown that intradermal (i.d.) application of RNActive® vaccines is able to confer protection against lethal influenza and rabies virus challenge infection in mice and induces protective levels of functional antibody responses against both viruses in domestic pigs. However, having been optimized for i.d. application, immunological responses upon intramuscular (i.m.) injection of these mRNA vaccine formulations remained less efficient as yet. Here, we describe an alternative formulation of RNActive® vaccines that is able to induce potent immune responses when applied intramuscularly using low doses (μg) of mRNA. Vaccination of mice with this RNActive® formulation encoding for influenza HA (hemagglutinin) or rabies G (glycoprotein) revealed an increase of both humoral and cellular immune responses, analyzed via functional antibody levels and ICS (intracellular cytokine staining) of T cells, respectively, compared to previous RNActive® formulations. Further experiments showed that this new vaccine formulation was able to induce potent and long lasting immune responses against influenza HA as well as high titers of rabies virus neutralizing antibodies in NHPs (non-human primates).|
Susanne Rauch works as a Scientist at CureVac where she is involved in the development of mRNA-based prophylactic vaccines. She has been trained as a Postdoc at King’s College London and as a PhD student at University Hospital Heidelberg where her work was focused on “The biology of different viruses such as HIV-1, MLV and herpesviruses.
Email: [email protected]
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