Analysis Of Avian Cell Proliferation By Flow Cytometry | 11143
Journal of Clinical & Cellular Immunology
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Cell proliferation for both T- and B-lymphocytes play an important role in the specific immunity against infectious diseases
and other mitogens. Techniques traditionally available for determining cell proliferation show a limited number of cell
divisions. Other techniques give indication of the overall division, without showing the history of the division. In addition, these
methods mostly use radioactivity of incorporated thymidine and bromodeoxyuridine into the DNA of mitotic lymphocytes. The
current paper will discuss a safe flow cytometric technique to measure cell proliferation
. This technique allows analysis of
the proliferation history such as the average number of divisions, the division index, the proliferation index and the percentage
of division. This technique is based on the use of carboxyfluoroscein succinimidyl ester stain (CFSE), a colourless and non-
fluorescent until it is cleaved by intracellular esterases to yield highly fluorescent CFSE. This fluorescence is retained by the cells
throughout development and meiosis, and so it is inherited by daughter cells. The divided cells can be displayed as a histogram of
FITC-CFSE staining against cell count. For analysis of data, a marker is set on the control sample, to include at least 98.5% of the
un-stimulated cells. The sensitivity of this protocol is critically affected by the original cell concentration, mitogen concentration,
incubation time, and the method of lymphocyte purification.
Hanan Al-Khalaifa graduated as honor student from Kuwait University with Zoology as major and Microbiology as minor. She joined KISR in 1996 as
a Research Assistant and was promoted to get her M.Sc. in Immuno-Parasitology from University of Manchester in 2003 and Ph-D in Immunology
from University of Reading in 2010, united Kingdom. She was the leader of three client funded projects, and three general activities. She participated
in eight client-funded and in-house projects. She authored and co-authored 25 refereed articles. She also authored and co-authored 20 papers
presented in international conferences and 7 progress and final in-house KISR (Kuwait Institute for Scientific Research) reports.
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