alexa Analysis Of Free Circulating DNA In Peripheral Blood | 19510
ISSN: 1747-0862

Journal of Molecular and Genetic Medicine
Open Access

OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

2nd International Conference on Genomics & Pharmacogenomics
September 08-10, 2014 DoubleTree by Hilton Hotel Raleigh-Brownstone-University, USA

Piotr Mieczkowski
ScientificTracks Abstracts: J Mol Genet Med
DOI: 10.4172/1747-0862.S1.002
Abstract
Free circulating DNA (fcDNA) in peripheral blood is a subject of increasing interest in the circles of medical diagnostic and cancer research. We decided to evaluate methods used for extraction and analysis of the fcDNA. The blood from patients was collected into three types of tubes: ACT, EDTA and Streck cell free DNA BCT. The cell free BCT tubes stabilize blood cells and do not allow for the uncontrolled release DNA or RNA to the plasma during the handling and preparation procedure. Extraction of DNA was performed using both column (Qiagen) and magnetic bead (SNOVA and OMEGA) protocols according to the manual provided by the manufacturer. The undergoing result of comparison methods for DNA purification from plasma will be presented. Extracted DNA was subjected for sequencing on Illumina Sequencing platform. We used three major methods for DNA sequence analysis - Whole Genome Sequencing, TruSeq DNA Capture and Amplicon protocols. We focused on Molecular Tag Amplicon sequencing strategy since this technology is most clinically relevant at this time. Therefore, data for a SNP targets amplified on PCR or Fluidigm Access Array platform used for cancer research will be presented during the meeting.
Biography
Piotr Mieczkowski has completed his PhD at the Institute of Biochemistry and Biophysics Polish Academy of Sciences and Postdoctoral studies at the University of North Carolina at Chapel Hill and Duke University. He is the Director of the High Throughput Sequencing Facility at UNC. He has published more than 56 papers in reputed journals. His work is focused around new applications for next generation sequencing technology, genome stability and mutagenesis.
image PDF   |   image HTML
 

Relevant Topics

Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2018-19
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri & Aquaculture Journals

Dr. Krish

[email protected]

1-702-714-7001Extn: 9040

Biochemistry Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals

Ronald

[email protected]

1-702-714-7001Extn: 9042

Chemistry Journals

Gabriel Shaw

[email protected]

1-702-714-7001Extn: 9040

Clinical Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Engineering Journals

James Franklin

[email protected]

1-702-714-7001Extn: 9042

Food & Nutrition Journals

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

General Science

Andrea Jason

[email protected]

1-702-714-7001Extn: 9043

Genetics & Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Materials Science Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Nursing & Health Care Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001Extn: 9038

Neuroscience & Psychology Journals

Nathan T

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

Ann Jose

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001Extn: 9042

 
© 2008- 2018 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version