Analysis Of Inducible Expression In Pharmaceutical Microorganisms | 62757
Pharmacoeconomics: Open Access
Like us on:
Our Group organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.
This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)
All submissions of the EM system will be redirected to Online Manuscript Submission System. Authors are requested to submit articles directly to Online Manuscript Submission System of respective journal.
An inducible enzyme is an enzyme that is expressed when specific compounds are added to the cells. While in general it is
inductively formed by the enzyme substrate, other compounds exhibiting induction of the enzyme were searched for. We
have focused on pharmaceutical actinomycetes. Particularly, Streptomyces genus has been industrially used for the production
of a lot of pharmaceutically important compounds, and therefore this genus should be used as a host strain for the formation of
such pharmaceuticals and enzymes involved in their biosynthesis. Previously, we have investigated microbial metabolism of nitrile
compounds. Particularly, we found that a nitrile, isovaleronitrile, can be a good inducer for the formation of nitrilase enzyme, which
degrades nitrile to the corresponding acid and ammonia; more than 35% of all soluble proteins are nitrilase in an actinomycete,
Rhodococcus strain. We also clarified the regulation mechanism of the nitrilase expression and identified the enzyme gene promoter
in the strain. Based upon its unique expression mechanism, developed an expression system using this enzyme gene promoter in
Streptomyces. Here, using actinomycetes, we searched for inducers, which can form a protein or enzyme inducibly. When amiderelated
compound was added into a culture medium in the actinomycete, a major protein band was observed on SDS-polyacrylamide
gel. More than 30% of all soluble protein in the cells, the protein was inductively overexpressed. This is very significant expression of
the protein, and therefore, this expression system could be useful for the development of a new expression vector for several industrial
Michihiko Kobayashi (BS, MS and PhD) is a Professor at University of Tsukuba (Japan). He graduated from Kyoto University (Agricultural Chemistry), Japan in 1985. He started his professional career in Kyoto University (1991-1999) as an Assistant Professor, Senior Lecturer and as an Associate Professor, respectively. In 1999, he became a Professor, and started the Lab of Molecular Microbial Bioengineering at University of Tsukuba. He has received many awards: (i) JSPS Prize (the Japan Society for the Promotion of Science Prize) (2004); (ii) High Technology Grand Prize “Special Prize” (2005); (iii) Prize for Science Technology (Research Category): The Commendation for Science and Technology by the Ministry of Education, Culture, Sports, Science and Technology, Japan (2006); (iv) SAJ Award (“the Society for Actinomycete Japan” Award) (2009); (v) Japan Bio-industry Association Award (2012); (vi) Japan Bioscience, Biotechnology and Agrochemistry Society Award (2015).