alexa Analysis Of Koumiss Microbiome In Akmola Region
ISSN: 2329-8901

Journal of Probiotics & Health
Open Access

Like us on:
OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

3rd International Conference and Exhibition on Probiotics, Functional & Baby Foods
September 23-25, 2014 Hotel Royal Continental, Naples, Italy

Kozhakhmetov Samat, Baiskhanova D, Tynybayeva I, Saduakhasova S, Shakhabaeva G, Kushugulova A, Nurgozhin T and Zhumadilov Zh
Posters: J Prob Health
DOI: 10.4172/2329-8901.S1.017
Abstract
Introduction: Koumiss is a low-alcohol product made from fermented mare?s milk. Today it is popular in Kazakhstan, Russia and other countries of the Central Asia, China and Mongolia. Natural mare?s milk is fermented in symbiosis of two types of microorganisms (lacto-bacteria and yeast). Koumiss microbial composition varies depending on the geographical, climatic and cultural conditions. For example Wu, R. and coauthors, on the basis of phenotypic characteristic from samples, isolated in Inner Mongolia and autonomous region of China identified following bacteria: L. casei, L. helveticus, L. plantarum, L. coryniformis subsp. coryniformis, L. paracasei, L. kefiranofaciens, L. curvatus, L. fermentum and W. kandleri . Studies of yeast composition in koumiss also showed significant differences. Thus, from 87 isolated yeast cultures to Saccharomyces unisporu related 48.3% of isolates, to Kluyveromyces marxianus (27.6%), Pichiamembranae faciens (15.0%), Saccharomyces cerevisiae (9.2%). Methods: To extract DNA from the koumiss, 1.8 ml of fermented milk was centrifuged to generate a pellet which was suspended in 450 μl of lysis buffer P1 from the Power food Microbial DNA Isolation kit (MoBio Laboratories Inc., USA). To determine the composition of the microflora was used to amplification a fragment of the gene 16S rRNA and ITS1. Plasmid library with target insertion were obtained on the basis of hight copy plasmid vectors produces high pGem-T. The definition of direct nucleotide sequence was performed by the method of Sanger using a set of ?BigDyeTerminanor v 3.1 Cycle sequencing Kit with automatic genetic analyzer ABI 3730xl (Applied Biosystems, USA). For the analysis were used the software package Informax Vector NTI Suite 9, Sequence Scanner v 1.0. Result: Studies showed, that in the most samples of koumiss isolated from Akmola region (The Central Kazakhstan) prevailed following bacteria species: Lactobacillus diolivorans, Lactobacillus acidophilus, L. casei, L. curvatus , yeast strain To r u l a (62.4%) and Saccharomyces cerevisiae (37.6%). Conclusions: Thus, there was conducted the first metagenomic research of koumiss in Kazakhstan, which showed significant differences in microbial composition of koumiss.
image PDF   |   image HTML
 
Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri & Aquaculture Journals

Dr. Krish

[email protected]

1-702-714-7001Extn: 9040

Biochemistry Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals

Ronald

[email protected]

1-702-714-7001Extn: 9042

Chemistry Journals

Gabriel Shaw

[email protected]

1-702-714-7001Extn: 9040

Clinical Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Engineering Journals

James Franklin

[email protected]

1-702-714-7001Extn: 9042

Food & Nutrition Journals

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

General Science

Andrea Jason

[email protected]

1-702-714-7001Extn: 9043

Genetics & Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Materials Science Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Nursing & Health Care Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001Extn: 9038

Neuroscience & Psychology Journals

Nathan T

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

Ann Jose

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001Extn: 9042

 
© 2008- 2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version