Muscadine grapes (Vitis rotundifolia), which are native to southeastern USA, are known to be potential source of anti
oxidant compounds. Our goal was to investigate pathways of various polyphenolic compounds synthesized during berry
development. The objective of this research is to investigate differentially expressed proteins involved in the biosynthesis of
these metabolites. Total proteins were isolated from muscadine berry samples from different developmental stages. Each protein
sample was labeled with iTRAQ 8 plex reagent and digested with trypsin. The peptides were then sequenced using the QSTAR XL
Mass spectrometer. Data refinement and analysis was performed in Peaks Studio 5.3 de novo sequencing assisted database search
for sensitive and accurate peptide identification. Proteome analysis revealed 94 differentially expressed proteins with > 5 fold
increase/decrease during berry development. Biological processes and metabolic pathways for these proteins were determined
using Blast2GO pathway analysis. The major enzymes like benzyl alcohol o- benzyl transferase involved in volatile production of
methyl anthranilate that may be responsible for distinctive flavor of muscadines and tropinone reductase involved in production
of tropane alkaloids that are used medicinally as anticholinergic agents were over expressed during berry development. Disease
resistant proteins PR10, PR-4, MLP and 14-3-3 like proteins were constitutively expressed during different stages of berry
development indicating unique disease resistance ability of muscadine grapes.
Ramesh Katam has completed his Ph. D from Mississippi State University and carried his postdoctoral studies from Florida A&M University (FAMU).
Currently he is Assistant Professor at Department of Biological Sciences at FAMU. He has published more than 20 research papers in peer
reviewed journals and serving in editorial board for several journals. He is specialized in functional proteomics and involved in research and teaching
proteomics to undergraduate and graduate students.
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