alexa
Reach Us +3225889658
Analytical Aspects And Biomedical Applications Of Selenium Speciation | 67071
ISSN: 2157-7064

Journal of Chromatography & Separation Techniques
Open Access

Like us on:

Our Group organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations
700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Analytical aspects and biomedical applications of selenium speciation

4th World Congress on MASS SPECTROMETRY

Nikolay Solovyev and Bernhard Michalke

Saint Petersburg State University, Russia Helmholtz Zentrum Muenchen, Germany

ScientificTracks Abstracts: J Chromatogr Sep Tech

DOI: 10.4172/2157-7064-C1-025

Abstract
Analytical challenges of selenium (Se) species determination, using hyphenated techniques, are discussed. Selenium (Se) is complicated element for the inductively coupled plasma (ICP) based techniques, owing to relatively low sensitivity and abundant interferences. Since emission lines of Se are insensitive, nearly exclusive mass spectrometry (MS) detection is used for Se speciation in biological samples. Nevertheless, selenium detection by ICP-MS is considerably less sensitive compared to most elements. This is due to rather high ionisation potential of Se, its natural isotope distribution and spectral interferences, originating from argon of the ICP as well as sample bulk elements. Noteworthy, all Se isotopes are interfered to certain extent. A primary isotope 80Se (abundance 49.61%) is totally interfered by argon dimer, whereas the less interfered 77Se has abundance of 7.63% only. Severe interferences for Se require for the implementation of sector-field MS or using of collision or dynamic reaction cell technologies for Se quantification and speciation. The best performance, in respect of limits of detection, is obtained for collision or dynamic reaction cells with oxygen/ hydrogen or methane as working gases. Additional analytical complications of Se speciation are related to separation and identification techniques, since biologically relevant Se-species diverse highly in their chemical nature: inorganic (selenite and selenate), organic, including seleno amino acids (selenocysteine, selenomethionine), low molecular weight species, specific selenoproteins (selenoprotein P, glutathione peroxidase, thioredoxin reductase etc.) and non-specific selenised proteins (e.g. selenised human serum albumin). The main biomedical applications of Se speciation are epidemiologic studies, selenium metabolomics and risk assessments studies.
Biography

Nikolay Solovyev completed his PhD at Saint Petersburg State University. He is an Analytical Chemist, and a Lecturer in Analytical Chemistry at Institute of Chemistry, Saint Petersburg State University. He has expertise is in “Clinical elemental analysis, speciation analysis and trace element metabolism”.

Email: [email protected]

Top