Immunome Research
Open Access

Antibodies are not required to a protective immune response against dengue virus elicited in a mouse encephalitis model

2^nd International Conference on Antibodies and Therapeutics

July 11-12, 2016 Philadelphia, USA

Jaime Henrique Amorim

Universidade Federal do Oeste da Bahia, Brazil

Posters & Accepted Abstracts: Immunome Res

Abstract :

Neutralizing antibodies have been considered prerequisite in the control of Dengue virus (DENV) infection. However, T-lymphocytes have also been shown to be important in a protective immune state. To investigate the contribution of both, humoral and cellular immune responses in DENV immunity, we used an experimental model in which a non-lethal DENV2 strain (ACS46) is used to prime Balb/C mice which develop protective immunity against a lethal DENV2 strain (JHA1). Primed mice generated envelope-specific antibodies and CD8+ T cell responses targeting mainly non-structural proteins. Immune sera from protected mice did not confer passive protection to naive mice challenged with the JHA1 strain. In contrast, depletion of CD4+ and CD8+ T-lymphocytes significantly reduced survival of ACS46-primed mice challenged with the JHA1 strain. Collectively, results presented in this study show that a cellular immune response targeting non-structural proteins are a promising way in vaccine development against dengue.

Biography :

Email: jhamorim@usp.br