Anti-inflammatory effect of Hedgehog’s skin extraction LPS-stimulated RAW 264.7 cells

3^rd International Conference and Exhibition on Traditional & Alternative Medicine

August 03-05, 2015 Birmingham, UK

Kang Hyun Leem and Myung Gyou Kim

Semyung University, Korea

Posters-Accepted Abstracts: Altern Integr Med

Abstract :

The hedgehog�s skin is one of the Korean medicines for hematochezia and hemorrhoids. It has been known to have the effect of calming the adverse-rising energy and stopping bleeding. This study investigated the anti-inflammatory effect of the hedgehog�s skin extract on RAW 264.7 cells. 70% ethanol extracts of the hedgehog�s skin were prepared and treated on LPS-stimulated RAW 264.7 cells for 24 hours. The production of nitric oxide was assayed in the culture medium of the cells and the expression of pro-inflammatory proteins such as inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX- 2), extracellular signal-regulated kinase 1/2 (ERK 1/2), phosphorylated ERK1/2, c-Jun N-terminal kinase 1/2 (JNK 1/2), and phosphorylated JNK 1/2 were detected by Western blot analyses. The extract of the hedgehog�s skin suppressed LPS-induced nitric oxide productions, and it reduced the expression of COX-2 and iNOS. Also, it inhibited the phosphorylation of ERK1/2 and JNK in LPS-stimulated RAW 264.7 cells. These experiments suggest that the extract of the hedgehog�s skin has an antiinflammatory activity through the regulation of the mitogen-activated protein kinase pathway in LPS-stimulated RAW 264.7 cells.

Biography :

Kang Hyun Leem is the Director of Department of Herbal Pharmacology, College of Korean Medicine, Semyung University.

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