Immunome Research
Open Access
ISSN: 1745-7580
+44-20-4587-4809
ISSN: 1745-7580
+44-20-4587-4809
Mohammad R Pourshafie, Mahdi Rohani, Nasrin Noohi and Malihe Talebi
Pasteur Institute of Iran, Iran
Iran University of Medical Sciences, Iran
Posters & Accepted Abstracts: J Immunome Res
In the present study, acute colitis was induced in Balb/c mice with oral administration of 2% DSS and the MPO activity and interleukin 8 production was assessed. For the determination of putative influence of 10 final selected strains in IL-8 production, HT-29 cells were seeded at a concentration of 2�?�?105 cells/ml in 24-well plates. The anti-inflammatory effect of the selected Lactobacillus strain was assessed in a chemically induced inflammatory bowel disease (IBD) with dextran sulfate sodium (DSS) in Balb/c mice. These three features included colon length, disease activity index and myeloperoxidase (MPO) activity in the middle section of colon tissue. Among the 20 Lactobacillus strains tested, 6 strains showed decreased proliferation by more than 20%. The most anti-proliferative effect was observed with L. plantarum 365 with an inhibition rate of 44.9�?±4.9%. No correlation was found between specific species of Lactobacillus strains and anti-proliferation Caco-2 cell line. None of the tested strains induced secretion of IL-8 in HT-29 cells by themselves. The results showed L. rhamnosus 195, L. plantarum 240 and L. plantarum 156 decreased LPS- induced IL-8 production by >50% (55.1, 54.8 and 50.6%, respectively). No significant difference in the reduction of LPS-induced IL-8 was found among the different species of Lactobacillus (p=0.0509). In the animal model used, we scored disease activity index (DAI) based on weight loss, fecal character and fecal occult blood. Administration of dextran sulfate sodium (DSS) shortened the colon length and increased DAI as expected. L. plantarum strains 42 and 156, L. rhamnosus strain 195 and L. brevis strain 165 significantly reduced DAI score in the bacteria treated mice. All Lactobacillus strains could significantly (p<0.01) prevent the shortening of colon. As expected, a high activity of myeloperoxidase (MPO) in the DSS group was observed compared to the control group (P<0.001).
Email: m_pour@pasteur.ac.ir