Biochemistry & Pharmacology: Open Access
Open Access
ISSN: 2167-0501
ISSN: 2167-0501
Mostafa M. El-Sheekh
Accepted Abstracts: Biochem & Pharmacol
Cultures of the blue green algae (cyanobacteria) Anabaena wisconsinense , Oscillatoria curviceps and Nostoc muscorum were isolated from fish farms and their antimicrobial effects were studied. Solvent extracts of exponential phase algae were screened for antimicrobial activity against different species of gram positive ( Lactobacillus sp . and Bacillus firmus ) and gram negative ( Aeromonas Hydrophila, Pseudomonas fluorescens and Pseudomonas anguilliseptica ) bacteria and the fungi ( Aspergillus niger and Saprolegnia parasitica ) which were all isolated from diseased fish. Maximum antimicrobialactivity was observed with ethanolic extract of O. curviceps against Lactobacillus sp. and Aeromonas Hydrophilia , whereas, methanol extracts of A. wisconsinense and O. curviceps had antibacterial effects against B. firmus, A. Hydrophila, P.fluorescens, P. anguilliseptica , and the fungi A. niger and S. parasitica . It was also observed that solvent extracts of A. wisconsinense when injected into Oreochromis niloticus which was already injected intraperitoneally with 2x106 CFU/ml, P. Anguilliseptica significantly decreased fish mortality from 50 to 12.5%. The cyanobacterium Nostoc muscorum exhibited antagonistic activity against gram-positive and gram-negative bacteria and filamentous fungi. The results indicated that the active substance produced maximally after 12 days of incubation in shaken culture at 35 0 C, at pH 8.0 in BG-11 medium. The antagonistic material was purified using thin layer chromatography. The compound showed maximum absorption at 240 nm. Infrared (IR) and nuclear magnetic resonance (NMR) indicated presence of γOH, γCH aromatic, γCH aliphatic, γC=N, γC=O, γC=C and C-O. Mass spectroscopy indicated that its molecular weight is 279. The results also indicated that the compound isophenolic compound.
Mostafa M. El-Sheekh has completed his Ph.D. in 1991 as channel system fellowship between Tanta University, Egypt and Goettingen University, Germany. He have done postdoctoral studies in Hungary, Czech Republic, Japan, Sweden, India and he was visiting Professor in different universities worldwide in Turkey, Azerbaijan, Morocco, Algeria. He organized and attended many international conferences and workshops in Spain, Algeria, Egypt, India and USA. He published more than 72 papers in local and international journals and there another 6 papers under reviewing and more than 10 papers under preparation. He has now 5 research projects. And he is supervising 18 Ph.D. and M.Sc. students in different universities in Egypt. He is reviewer and editor in more than 45 international journals.