Journal of Bacteriology & Parasitology

Anti-oxidative and neuro-protective potentials of a novel mangrove soil action-bacterium, Streptomyces antioxidans sp. nov. MUSC 164T

7^th International Conference on Bacteriology and Infectious Diseases

June 04-05, 2018 Osaka, Japan

Hooi-Leng Ser, Kok-Gan Chan, Bey-Hing Goh and Learn-Han Lee

Monash University Malaysia Campus, Malaysia
University of Malaya, Malaysia
University of Phayao, Thailand

Posters & Accepted Abstracts: J Bacteriol Parasitol

Abstract:

Over the years, exploring new taxa remains as one of the successful strategies which lead to discovery of therapeutic agents. As the most prolific producer of bioactive compounds, members of Streptomyces genus are well known in producing pharmaceutically relevant activities such as anti-inflammatory, antimicrobial, antioxidant activities. During a screening program for bioactive Streptomycetes, strain MUSC 164T was recovered from a mangrove soil located in east coast of Peninsular Malaysia. A polyphasic approach revealed that strain MUSC 164T possessed a spectrum of phylogenetic and chemotaxonomic properties consistent with those of the members of the genus Streptomyces. Phylogenetic analysis identified that closely related strains for MUSC 164T as Streptomyces javensis NBRC 100777T (99.6% sequence similarity), Streptomyces yogyakartensis NBRC 100779T (99.6%) and Streptomyces violaceusniger NBRC 13459T (99.6%). The DNA-DNA relatedness values between MUSC 164T and closely related type strains ranged from 23.8±0.3% to 53.1±4.3%. Based on the polyphasic study of MUSC 164T, it is concluded that this strain represents a novel species, for which the name Streptomyces antioxidans sp. nov. is proposed. As an attempt to explore its bioactive potential, the methanolic extract of MUSC 164T was subjected to several antioxidant assays prior to in vitro neuro-protective screening against hydrogen peroxide (H2O2). The extract of MUSC 164T showed potent anti-oxidative and neuro-protective activities against hydrogen peroxide. The chemical analysis of the extract revealed that the strain produces pyrazines and phenolic-related compounds that could explain for the observed bioactivities. Moreover, in-depth investigations focusing on the genome of MUSC 164T suggested further bioactive potential of the strain, including production of desferroxamine B. In short, these promising findings not only highlight the importance of mangrove forest for the discovery of novel streptomycetes in Malaysia, but also suggest potential exploitation of these strains for development of clinically useful drugs against cancer.

Biography :

Hooi-Leng Ser is currently pursuing PhD at Monash University Malaysia Campus. Currently, her research focus includes identification of novel Streptomyces species derived from mangrove forests before investigating bioactive potential of their secondary metabolites (e.g. antioxidant, anticancer, neuro-protection using human cell lines). She has 2 patents and published 20 ISI-cited journal articles with 12 articles published in Q1 journals.
Email:ser.hooi.leng@monash.edu

Email:hooileng_ser@y7mail.com