alexa Antiproliferative Activity Of Bacterial Proteins Against MDA-MB-231 Human Breast Adenocarcinoma And Hepg2 Human Hepatocellular Carcinoma Cells
ISSN: 1948-5956

Journal of Cancer Science & Therapy
Open Access

Like us on:
OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Share This Page

Additional Info

Loading
Loading Please wait..
 

4th World Congress on Cancer Science & Therapy
October 20-22, 2014 DoubleTree by Hilton Hotel Chicago-North Shore Conference Center, USA

Asvene K Sharma, Pratibha Vats and Partha Roy
Accepted Abstracts: J Cancer Sci Ther
DOI: 10.4172/1948-5956.S1.035
Abstract
Cancer is one of the most dangerous human diseases, expected to kill about 13.3 million people annually by 2030. Protein extracts of Lactobacillus sps (MTCC 4184, 9496 and 10093), Bacillus cereus (MTCC 7166, 9017 and 10202), Serratia marcence (MTCC 7641, 7298 and 7729), Shigella flexneri (MTCC 1457 and 9543) were evaluated for anticancer potential against human cancer cell lines MDA-MB-231 and Hep-G2. Inhibition of cancer cells proliferation and apoptosis was confirmed by MTT Cell Viability Assay, Fluorescent Staining, Caspase-3 activity, DNA fragmentation and cell cycle analysis by Flowcytometry. The results showed that protein extract of Lactobacillus sps (MTCC 4184), Bacillus cereus (MTCC 10202) and Shigella flexneri (MTCC 9543) inhibited both MDA-MB-231 and HepG2 cancer cell lines. Protein extract of Lactobacillus sps (MTCC 9496), Serratia marcence (MTCC 7298 and 7729) and Bacillus cereus (MTCC 7166) inhibited growth of MDA-MB-231, while no effect on HepG2 was observed. Protein extract of Lactobacillus sps (MTCC 10093), Bacillus cereus (MTCC 2763), Serratia marcence (MTCC 7641) and Shigella flexneri (MTCC1457) were none effective to any of the cell lines. The cancer cell mortality was observed from 52% to 71% + 2%. Fluorescent staining and increased Caspase 3 activity confirmed by western blotting, DNA fragmentation and detection of cell cycle arrest by Flow cytometry suggested that apoptosis was the major mechanism in inhibition of MDA-MB-231 human breast adenocarcinoma and Hep-G2 human hepatocellular carcinoma cells. The study confirms the anticancer potential of bacterial proteins which may constitute valuable candidates for development of novel anticancer drugs.
Biography
Asvene K Sharma has completed his PhD in Molecular Biology from CCS University Meerut. He is working as Young Scientist Fellow at Department of Biotechnology, Indian Institute of Technology-Roorkee, India and is working as a Principal Investigator for his DST Young Scientist Award Research Project.
image PDF   |   image HTML
 

Relevant Topics

Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

 
© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
adwords