Application Of A Recombinant Hybrid Mussel Adhesive Protein As A Functional Cosmetic Ingredient | 85232
Journal of Tissue Science & Engineering
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Application of a recombinant hybrid mussel adhesive protein as a functional cosmetic ingredient
Joint Event on 9th International Conference and Exhibition on Tissue Engineering and Biobanking & 9th International Conference and Exhibition on Tissue Science and Regenerative Medicine
Ye-Eun Yoon, Yoonjin Lee, Kyung-Bae Pi, Sung-Gil Park, Sang Uk Chung, Jong Ho Park and Hye-Won Lim
Cosmocos Corporation, Republic of Korea
Incheon Business Information Technopark, Republic of Korea
Advanced Biotech Inc., Republic of Korea
Skin Research Center, Republic of Korea
Shebah Biotech Inc., Republic of Korea
Marine mussels produce and secrete adhesive proteins that allow themselves to attach in rough marine environments. Currently, six mussel adhesive proteins (fp-1 through fp-6) have been identified from the adhesive plaques of mussels. Mussel adhesive proteins have been considered as a desirable source of water-resistant bioadhesives which can also be used as a useful ingredient in cosmetics. This study aimed to construct a recombinant hybrid mussel adhesive protein, fp-13151, in order to get convenient and economical production and enhance the adhesive capability of the mussel proteins, and evaluate its potential as a functional cosmetic ingredient. The hybrid gene was constructed to contain the parts of fp-1/fp-3/fp-1/fp-5/ fp-1in a sequential order using recombinant DNA techniques. fp-13151 was purified from the corresponding overexpressed E. coli cells using affinity chromatography. fp-13151 did not exhibit cytotoxicity and irritability to the skin. fp-13151 at 50 μg/ ml augmented the synthesis of collagen 1.63-fold over that of the non-treated control in HaCaT cells, implying its anti-wrinkle activity. On the contrary, fp-13151 diminished the levels of matrix metalloproteinase (MMP-1), also known as interstitial collagenase, in a concentration-dependent manner. It could marginally inhibit elastase activity in an in vitro experiment. fp13151 was able to inhibit both monooxygenase and oxidase activities of mushroom tyrosinase in a concentration-dependent manner, suggesting its skin whitening activity. It was also found to contain an antioxidant activity, when an ABTS radical scavenging capacity assay was used. In a pilot-scale clinical trial, the essence containing fp-13151 significantly reduced the wrinkle parameters tested in the participants after both 4- and 8-week treatment, compared to the control group. Taken together, fp-13151 possesses skin beneficial properties, such as antioxidant, whitening and anti-wrinkle activities, in addition to its peculiar adhesive character. These findings suggest that fp-13151 has potential as an effective ingredient in the manufacture of functional cosmetics.
Ye-Eun Yoon received her MS degree from the Department of Chemical and Biomolecular Engineering, Yonsei University, Seoul, Korea. Her Master’s thesis is entitled “Development of adhesive/multi-layered scaffolds for tissue adhesives and gene delivery vehicles”. She has already published several peer-reviewed research papers in reputed journals and won an American Chemical Society (ACS) Editors’ Choice Award in 2016. Currently, she works as a Research Scientist at Research & Development Center, Cosmocos Corporation, a branch of Korea Tomorrow and Global (KT&G), in Korea.
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