Living cells of
(MTCC-1483) were entrapped in calcium alginate beads for the α-amylase production.
There was maximum production after 24 hrs at pH 6.5 when 1X 10
cells /ml immobilized in 2 % sodium alginate
was inoculated in the production medium containing 2% starch and 1.5% calcium carbonate as inducer. Response surface
methodology (RSM) in conjunction with central composite design (CCD) was performed to optimize the amylase purification
parameters by ethaol extraction methods. Maximum purification fold (1.6) and % recovery (77%) was achieved by using 90%
acetone, pH 4, temp. 4
C and retention time of 1 hr. To increase the purification level further extraction was carried out by ethanol
Solvent using RSM. The maximum purification fold (13) and % recovery (23%) was achieved by using 10% ethanol, pH 6.5, temp.
C and retention time of 12 hrs. The purified cellulase has molecular mass of 32.5 kDa, and optimal pH and temperature at 6.5
C, respectively. The purified cellulase activity was inhibited by Na
Tween 80 and EDTA. Amylase extracted by
ammonium sulphate precipitation has specific activity 24.68 IU/mg of protein, optimum pH 7 and temperature 60
mg/ml and V
Jagdish Singh has completed his Ph.D. at the age of 28 years from Punjabi University Patiala Punjab India. Presently he is the head of Department
in The Biotechnology Department In the, a premier organization Mata Gujri college Fatehgarh Sahib Punjab. He has published 08 papers in reputed
journals and serving as member of different board of studies. He is running two research projects granted by central government Funding agencies.
He has guided more than 75 postgraduate research projects
Peer Reviewed Journals
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals