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|Xiaoyi Wang and Richard J Payne|
|The University of Sydney, Australia|
|Posters & Accepted Abstracts: J Proteomics Bioinform|
|An efficient synthesis of a p-methoxybenzyl(PMB)-protected β-selenoleucine derivative from commercially available Garner’s aldehyde is described as well as its incorporation into the N-termini of peptides via Fmoc-solid phase peptide synthesis (SPPS) is discussed. Simple mixing of these selenopeptides with peptides containing C-terminal selenoester functionalities in aqueous buffer rapidly provides native amide bonds in 5 minutes to 3 hours in high yields without the use of any other reagents or additives. Following the ligation event, an in situ deselenization rapidly affords native peptide product without any hydroxylation by-product. This method has been applied to synthesize an 83-mer human cytomegalovirus protein, UL22A, which selectively binds RANTES (regulated upon activation, normal T cell expressed and secreted) with high affinity. This chemistry now serves as a promising new technology for the rapid one-pot synthesis of other natural proteins or biological and therapeutic interest.|
Xiaoyi Wang (Eileen) is a 3rd year PhD candidate at the University of Sydney and is supervised by Prof. Richard J Payne. She has her expertise in Protein Chemistry regarding Fmoc solid phase peptide synthesis, peptide ligation and stapled peptides. She received an MRes degree in Drug Discovery and Development from Imperial College London in Prof. Ed Tate’s group. She has worked for Pfizer and other three big Chinese pharmaceutical companies (Hengrui, Hansoh and Kanion) as an Analytical Chemist, Medicinal Chemist, Marketing Intern and Middle-Scale Manufacturing Intern.
Email: [email protected]
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