alexa Application Of β-seleno-leucine In One-pot Ligation-deselenization Chemistry
ISSN: 0974-276X

Journal of Proteomics & Bioinformatics
Open Access

Like us on:
OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Share This Page

Additional Info

Loading
Loading Please wait..
 

8th International Conference on Proteomics and Bioinformatics
May 22- 24, 2017 Osaka, Japan

Xiaoyi Wang and Richard J Payne
The University of Sydney, Australia
Posters & Accepted Abstracts: J Proteomics Bioinform
DOI: 10.4172/0974-276X-C1-098
Abstract
An efficient synthesis of a p-methoxybenzyl(PMB)-protected β-selenoleucine derivative from commercially available Garner’s aldehyde is described as well as its incorporation into the N-termini of peptides via Fmoc-solid phase peptide synthesis (SPPS) is discussed. Simple mixing of these selenopeptides with peptides containing C-terminal selenoester functionalities in aqueous buffer rapidly provides native amide bonds in 5 minutes to 3 hours in high yields without the use of any other reagents or additives. Following the ligation event, an in situ deselenization rapidly affords native peptide product without any hydroxylation by-product. This method has been applied to synthesize an 83-mer human cytomegalovirus protein, UL22A, which selectively binds RANTES (regulated upon activation, normal T cell expressed and secreted) with high affinity. This chemistry now serves as a promising new technology for the rapid one-pot synthesis of other natural proteins or biological and therapeutic interest.
Biography

Xiaoyi Wang (Eileen) is a 3rd year PhD candidate at the University of Sydney and is supervised by Prof. Richard J Payne. She has her expertise in Protein Chemistry regarding Fmoc solid phase peptide synthesis, peptide ligation and stapled peptides. She received an MRes degree in Drug Discovery and Development from Imperial College London in Prof. Ed Tate’s group. She has worked for Pfizer and other three big Chinese pharmaceutical companies (Hengrui, Hansoh and Kanion) as an Analytical Chemist, Medicinal Chemist, Marketing Intern and Middle-Scale Manufacturing Intern.

Email: [email protected]

image PDF   |   image HTML
 

Relevant Topics

Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

 
© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
adwords