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Automated Microinjection Of Cell-polymer Suspensions In 3D ECM Scaffolds For Highthroughput Quantitative Cancer Invasion Screens | 5114
ISSN: 2157-7013

Journal of Cell Science & Therapy
Open Access

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Automated microinjection of cell-polymer suspensions in 3D ECM scaffolds for highthroughput quantitative cancer invasion screens

International Conference on Emerging Cell Therapies

Jan de Sonneville

ScientificTracks Abstracts: J Cell Sci Ther

DOI: 10.4172/2157-7013.S1.002

Abstract
C ell spheroids (CS) embedded in 3D extracellular matrix (ECM) serve as in vitro mimics for multicellular structures in vivo. We describe a method where cell-polymer suspensions are microinjected as droplets into collagen gels and CS formation occurs within hours for a broad range of cell types. We have automated this method to produce CS arrays in fixed patterns with defined x-y-z spatial coordinates in 96 well plates and applied automated imaging and image analysis algorithms. Low intra- and inter-well variation of initial CS size and CS expansion indicates excellent reproducibility. Distinct cell migration patterns can be visualized and manipulated
Biography

Jan de Sonneville done Bachelors in Electrical Engineering, Delft University of Technology (TUDelft), Master in NanoScience (Applied Physics), given as joint program by TUDelft and Leiden University, the Netherlands (2006). PhD on the development of four novel research methods for Cell Biology, thesis title: ?Reinventing microinjection, new microfluidic methods for cell biology? (2011). Founded Life Science Methods BV to sell Automated Microinjection Systems for high throughput screening using cell spheroids and zebrafish embryos (2011).

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