alexa Automated Open-access Liquid Chromatography High Resolution Mass Spectrometry To Support Medicinal Chemistry Projects | 67066
ISSN: 2157-7064

Journal of Chromatography & Separation Techniques
Open Access

Like us on:

OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations
700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

4th World Congress on MASS SPECTROMETRY

Alberto Fontana, Laura Iturrino, Corens, David and Antonio L Crego
Janssen Research & Development, Spain
Janssen Research & Development, Belgium
University of Alcalá, Spain
ScientificTracks Abstracts: J Chromatogr Sep Tech
DOI: 10.4172/2157-7064-C1-025
Abstract
The need of a continuous productivity increase in the pharmaceutical industry motivated the development of new open-access tools on many analytical techniques. On LC, these platforms have been commonly used with low-resolution MS detectors while they have been reported with very limited automation on high-resolution MS spectrometers. Typically, samples with concentration ranges around 0.1 mg/mL are prepared by medicinal chemists for reaction monitoring and for UV purity assessment. Together with the evolution of the instrumentation, new software packages to automatically process HRMS data have appeared. These systems are typically run by specialists and developed for biological samples with compounds in concentrations of μg/mL range. In this work, the development of a fully automated open-access methodology on a UHPLC-DAD/ESI-QTOF system is reported, being applied to medicinal chemistry discovery projects from Janssen R&D. With the first MS conditions applied, accuracies <±1 mDa were not achieved in 20-30% of the cases in ESI+ due to oversaturation. After optimization of MS conditions, a new ESI+ method was implemented. This method has been used by chemists for more than 2,000 compounds from structurally diverse chemical series with a success >98% in exact mass determinations. For the other 2% of the samples, specific conditions are used. Upon training, chemists log-in samples and interpret the results by themselves in 90-95% of the cases. To our knowledge, for the first time, full automation has been achieved for an open-access LC-DAD-HRMS platform developed for end-users in a pharmaceutical environment.
Biography

Alberto Fontana completed his Master of Science in Chemistry from University Autónoma of Madrid. He joined Janssen Research & Development (Spain) in 1998. Since 2001, he works as Scientist in the analytical sciences team. He is focused on analytical LCMS, GCMS and on preparative LC, developing new methodology for Medicinal Chemistry groups, mainly within Neuroscience area. He is currently pursuing his PhD at University of Alcalá de Henares, Madrid. He is the main Author of one publication and Co-author of another five papers. He has presented several posters with his research work on LCMS at different international congresses.

Email: [email protected]

image PDF   |   image HTML
 
Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2018-19
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri & Aquaculture Journals

Dr. Krish

[email protected]

+1-702-714-7001Extn: 9040

Biochemistry Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals

Ronald

[email protected]

1-702-714-7001Extn: 9042

Chemistry Journals

Gabriel Shaw

[email protected]

1-702-714-7001Extn: 9040

Clinical Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Engineering Journals

James Franklin

[email protected]

1-702-714-7001Extn: 9042

Food & Nutrition Journals

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

General Science

Andrea Jason

[email protected]

1-702-714-7001Extn: 9043

Genetics & Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Materials Science Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Nursing & Health Care Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001Extn: 9038

Neuroscience & Psychology Journals

Nathan T

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

Ann Jose

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001Extn: 9042

 
© 2008- 2018 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version