Journal of Clinical Toxicology
Open Access

Behavioral, morphological deformities and biomarkers of oxidative damage as indicators of sublethal cypermethrin intoxication on the tadpoles of D. melanostictus

International Toxicology Summit & Expo

November 26-28, 2012 Hilton San Antonio Airport, USA

Muniswamy David

AcceptedAbstracts: J Clinic Toxicol

Abstract :

C oncerns have been raised that the amphibian larval stages are particularly at risk and may be vulnerable to adverse effects of pesticides. The present study reports acute toxicity of cypermethrin at 24, 48, 72 and 96 h through static renewal bioassay test for Duttaphrynus melanostictus . The LC 50 values were 5.15, 4.55, 3.95, and 3.34μg/L for 24, 48, 72, and 96 h respectively. At sublethal concentration (0.33μg/L) behavioral, morphological and biochemical changes were studied. The behavioral and morphological anomalies observed in the present study are typical signs of cyano pyrethroid poisoning. Significant changes were observed in total, soluble, and structural proteins. The depletion of all the protein fractions observed in this investigation led to progressive protein oxidation and catabolism of proteins. Decreased protein level has resulted in a marked elevation of free amino acid levels at all time intervals. The induction of catalase, glutathione-S-transferase activities and elevation in the levels of hydrogen peroxide, reduced glutathione, and malondialdehyde eventually lead to oxidative damage of biomolecules, showing that the generation of reactive oxygen species and oxidative stress are involved in the toxicity induced by cypermethrin. Indicating increased susceptibility of tadpoles. Thus, an exposure to cypermethrin at sublethal concentration had catastrophic effect on tadpoles of D. melanostictus

Biography :

Muniswamy David completed his education (M.Sc., M.Ed., M.Phil., Ph. D.) at Andhra Pradesh, India. After short service in State Fisheries Department at Andhra Pradesh, later he entered teaching profession at Karnatak University, Dharwad, Karnataka, India. Dr. David significantly contributed in the field of aquatic toxicology, Ecotoxicology and fishery biology. He is the member of editorial board for many peer reviewed research journals. He published more than 70 research papers in peer reviewed national and international journals. He is guiding many research scholars for their Ph.D. and M.Phil degrees and he is handling many funded research projects