Binding mode of various DNA binding molecules and natural DNA under cell mimic condition

10^TH ANNUAL Chemistry & Mass Spectrometry Congress

OCTOBER 18-19, 2017 OSAKA, JAPAN

Ye Sol Oh, Jin Ha Park, Sung Wook Han, Seog K Kim and Young-Ae Lee

Yeungnam University, Republic of Korea

Posters & Accepted Abstracts: Chem Sci J

Abstract :

Meso-tetrakis (N-methyl pyridinium-4-yl) porphyrin (TMPyP) intercalates between the base-pairs of DNA at a low [TMPyP]/[DNA base] ratio in aqueous solutions and molecular crowding conditions, which is induced by the addition of poly Ethylene Glycol (PEG). Studied DNA binding drugs, including TMPyP, 9-AA (aminoacridine), EB (Ethidium Bromide) and DAPI (4�, 6-diamidino-2-phenylindole) showed similar binding properties in the presence or absence of PEG molecules which is examined by circular and linear dichroism. According to the LDr (reduced Linear Dichroism) results of the binding drugs examined in this work, PEG molecules induced no significant change compared to their binding properties in aqueous buffering systems. These results suggest that the transition moments are not expected to be perturbed significantly by PEG molecules. In this study, the experimental conditions of PEG 8000 were maintained at 35% (v/v) of total reaction volume, which is equal to the optimal molar concentration (0.0536 M as final concentration for PEG 8000) to maintain suitable cell like conditions. Therefore, there was no need to focus on the conformational changes of the DNA helical structure, such as forming irregular aggregate structures, induced by large quantities of molecular crowding media itself at this stage.

Biography :

Ye Sol Oh has completed her undergraduate studies and currently pursuing Masters from Yeungnam University, Republic of Korea.
Email:snfl3671@naver.com