alexa Bioanalytical Method Development, Validation And Pharmacokinetics Study Of 5-Fluorouracil Loaded Nanoparticles | 68372
ISSN: 2169-0138

Drug Designing: Open Access
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4th Annual Congress on Drug Discovery & Designing

Saurabh Srivastava
King George’s Medical University, India
Posters & Accepted Abstracts: Drug Des
DOI: 10.4172/2169-0138-C1-014
Abstract
Background: 5-fluorouracil (5FU) is a chemotherapeutic agent against different types of cancer. 5FU loaded with nanotechnology can enhance efficacy over conventional drawback of 5-FU, such as short half-life, toxicity, low bioavailability and non-selective action. Pharmacokinetic profile of this advanced nano-formulation is needed to correlates with overall ADME (absorption, distribution, metabolism and excretion) process. Purpose: The purpose of this study is to develop HPLC-UV method and validate its performance in expression of specificity, precision, sensitivity, accuracy and stability of the developed 5-fluorouracil nanoparticles (5-FUNPs) and correlate and collect the valuable pharmacokinetics data. Methodology: 5-FUNPs were formulated with polymer poly lactic co-glycolic acid with oil-in-water/solvent evaporation. Characterizations of nanoformulation were performed which included particle size and stability studies. Analytical method was developed and validated from HPLC-UV and applied to pharmacokinetic parameters. Results: The calibration curve plotted for 5-FUNPs was linear at 267 nm. The lower limit for the quantification was found 10.13 ng/mL. The size of 5-FUNPs was between 137±0.97 to 193±0.93 nm and zeta potential between 0.27±0.08 to 0.29±0.07 mv on the side of positively charged. The highest peak for drug concentration, Cmax was 3.235±0.78 mg/L at highest time point, Tmax 7.21±2.52 hrs. The AUC(0-96) & AUC(0-∞) showed 8.89±4.98 mg/L-h and 9.57±3.77 mg/L-h respectively and t1/2 was 22.98±3.73 hrs. Conclusion: The results show a simple, specific, sensitive and stable HPLC-UV method for the quantitative determination of 5-FUNPs in plasma and successfully applied to the pharmacokinetic study after oral administration in rats.
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