Journal of Drug Metabolism & Toxicology
Open Access

Biomolecules oxidation and antioxidant enzymes response as a result of injection of oxidative stressors into 5th instar of Schistocerca gregaria

International Conference on Toxicogenomics and Drug Monitoring

August 25-27, 2015 Valencia, Spain

Eman Alaaeldin Adbelfattah, Taha Mohamed Bassal and Moataza Ahmed Abdel-Ghani Dorrah

Cairo University, Egypt

Scientific Tracks Abstracts: J Drug Metab Toxicol

Abstract :

Oxidative stressors may cause oxidative stress by generation of reactive oxygen species including free radicals and non free radicals, and therefore enhance oxidative damage to macromolecules. The aim of this work is not only to measure of products of ROS-oxidized macromolecules, but also to determine activity of antioxidant enzymes that were measured using spectrophotometric method and alkaline comet assay. The results show that each three stressors that were injected separately lead to formation of lipid peroxidation, protein carbonylation, and DNA strand breaks. Antioxidant response mechanism of the cells, as indicated from antioxidant enzymes level, lead to elevation of antioxidant enzymes superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), and glutathione reductase (GR) when compared to control (pvalue<0.05).

Biography :

Eman Alaaeldin Abdelfattah has graduated from Faculty of Science, Cairo University, Egypt. She is an instructor at Entomology Department, Faculty of Science, Cairo University, Egypt.

Email: eman.alaaeldein@gmail.com