tandard? cell cultivation is still performed under so called static conditions (5% CO2 at 37oC) incubators in plastic well plates
in ambient atmosphere. Static cultures are insufficient to mimic the in vivo conditions, thus cultivations should be performed
in a bioreactor where oxygen, pH and the transport of nutrients and metabolic waste in the tissue microenvironment can easily
be controlled. For the cultivation of mammalian cells including human stem cells from different sources several bioreactors
are already available. These include systems for the expansion and guided differentiation of cells under well controlled and
reproducible conditions. The cells within the bioreactors are growing on surfaces and/or biomaterials such as microcarriers or
porous structures so that different dynamic systems are used (e.g. stirred tank bioreactors, rocking platforms, perfusion systems,
rotating bioreactors). Furthermore many systems have been developed for the applications of biomechanical stimulation e.g.
shear stress via fluid flow, strain, compression, torsion. Physiological ?training? is of special interest in stem cell differentiation
and towards functional 3 D tissues which also includes the cultivation under ?hypoxic? (less than 20% oxygen) conditions.
Moreover, recently disposable bioreactors and bioprocess strategies for automatization have been developed enabling GMP
conform production of cells for cell therapy and tissue engineering applications.
The lecture shall give an update on current research activities within the field of bioreactor developments including the
integration of sensors for the production of clinical relevant cell quantity and quality for cell based therapies and tissue engineering
Cornelia Kasper completed her PhD 1998 from Leibniz University of Hannover (Germany) and her habilitation in 2007 at the Institute for Technical
Chemistry at the Leibniz University of Hannover. She was appointed as full university professor for ?Biopharmaceutical Production and Technology?
at University of Natural Resources and Life Science (BOKU) in Vienna (Austria) at the department of biotechnology. She has published more than
60 papers in reputed journals and several book chapters and is editor within the series ?Advances in Biochemical Engineering and Biotechnology?
(Springer, 3 volumes already published, 3 more in preparation)
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