Journal of Drug Metabolism & Toxicology
Open Access

blaNDM-1 possessing Escherichia coli and Klebsiella pneumoniae isolates exhibiting multidrugresistant and pandrug-resistant phenotypes in Northeast India

World Congress and Exhibition on Antibiotics

September 14-16, 2015 Las Vegas, USA

Arijit Bora

Gauhati University, India

Posters-Accepted Abstracts: J Drug Metab Toxicol

Abstract :

Increasing reports on New Delhi metallo-�?²-lactamase-1 (NDM-1) producing Enterobacteriaceae, particularly Escherichia coli and Klebsiella pneumoniae constitute a serious threat to global health. Although, NDM-1 was first reported in 2009 in a Swedish patient previously admitted to an Indian hospital, till the data on the NDM-1 producing Enterobacteriaceae in Indian hospital is limited due to constrained resources. Therefore, the present study was designed to evaluate the incidence of blaNDM-1 gene in E. coli and K. pneumoniae isolates at a tertiary care referral hospital in Northeast India. A total of 412 consecutive, non-duplicate isolates of E. coli (n=221) and K. pneumoniae (n=191) were recovered from various clinical samples. On the basis of their reduced susceptibility to meropenem or ertapenem, 55 (24.88%) E. coli and 52 (27.22%) K. pneumoniae were screened for detection of blaNDM-1 by PCR. All screened isolates were found to be positive for blaNDM-1. Each of the blaNDM-1 possessing isolates of E. coli and K. pneumoniae was also found to be positive for one or more additional bla genes, such as blaTEM, blaSHV, blaCTX-M and blaAmpC. All the blaNDM-1 possessing isolates were â�?�?multidrug-resistantâ�? as well as 56.36% E. coli 63.46% of K.pneumoniae isolates with blaNDM-1 were â�?�?pandrug- resistantâ�?. In addition, few of the blaNDM-1 positive isolates showed reduced susceptibility to tigecycline and colistin, which extremely limits the treatment options for infections cause by NDM-1-positive isolates.

Biography :

Arijit Bora has completed his PhD from Department of Biotechnology, Gauhati University, Assam, India in association with Department of Microbiology, Sanjay Gandhi Post Graduate Institute of Medical Sciences, Lucknow, India. He has more than 10 years of academic and laboratory experiences in the field of Medical Microbiology. Presently, he is working as a scientific officer in the Department of Applied Sciences, GUIST, Gauhati University, Assam, India. He has published 09 papers in reputed journals and presented his research works in two international conferences.

Email: arijitbora07@gmail.com