alexa Byproducts As Profitable Alternative For Antimicrobial Peptide Production And Its Application In Bacterial Nanocellulose
ISSN: 2157-7439

Journal of Nanomedicine & Nanotechnology
Open Access

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3rd International Conference on Nanotek & Expo
December 02-04, 2013 Hampton Inn Tropicana, Las Vegas, NV, USA

Angela Faustino Jozala
Accepted Abstracts: J Nanomed Nanotechnol
DOI: 10.4172/2157-7439.S1.015
Abstract
The use of waste as culture media promotes economical advantages, since it can reduce environment pollution and stimulates new researches aiming science sustainability. Bacterial nanocellulose (BC) production using waste as culture media is a novelty, stimulating scale-up for industrial production and extended applications. The incorporation of antimicrobial, such as nisin, in bacterial cellulose has a wide applicability in pharmaceutical, medical, chemical, cosmetic, food and other areas. Nisin is a natural antimicrobial peptide, commonly used as food preservative; being effective at controlling a broad range of Gram- positive bacteria, including the multidrug-resistant pathogen. Evaluation of BC production and nisin activity after incorporation in the BC membranes were studied. BCs were submersed in a solution of 250 μg/mL nisin (Sigma?), in phosphate buffer saline (PBS) pH 4.5. After absorption, BCs were kept at 4oC and the nisin activity was determined, by agar diffusion assay with L. sakei as bioindicator, in several periods, up to 45 days. Results demonstrated that BC membranes have the ability to incorporate nisin after 4 hours. Nisin activity was higher in BC membranes, although only 43% of initial proteins were transferred into the membranes. The stability results indicated that nisin activity in the BC membranes was maintained up to 45 days of storage. Our studies highlighted the importance of an effective antimicrobial system able to assure safety and stability to pharmaceutical and medical products. On a near future, the BC membranes combined with nisin will be tested against Gram-positive and Gram- negative microorganisms, and its cytotoxicity effects
Biography
Angela Faustino Jozala has completed her Ph.D. at the age of 29 years at School of Pharmaceutical Science, University of S?o Paulo, Brazil. At the moment she is participating a postdoctoral program at the same School. She has been working with scientific researches since 2000 and as long of the time, she acquired knowledge for develop researches in industrial microbiology, biomaterials and biotechnology fields. Until the present, she published 18 scientific articles in indexed journals
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