alexa Candida Albicans, Comparative SAP4-6 Gene Expression From HIV Positive Patients With Oral Candidiasis And Commensal In Healthy Individuals
ISSN: 2471-9315

Applied Microbiology: Open Access
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12th International Congress on Microbial Interaction and Applications of Beneficial Microbes
July 17-18, 2017 Munich, Germany

Vita Meylani, Langkah Sembiring and Tri Wibawa
Siliwangi University, Indonesia
Gadjah Mada University, Indonesia
Posters & Accepted Abstracts: Appli Micro Open Access
DOI: 10.4172/2471-9315-C1-006
Abstract
Gene expression of SAP4-6 based on the ability of detection of mRNA was observed in Candida albicans isolates from HIVpositive patients with oral candidiasis and commensal healthy people. Members of the species of C. albicans strains selectively isolated from both sources (habitat) using CHROMagar medium. Isolates were then induced to express SAP4-6 by using gene inducers media SAP4-6. Analysis of gene expression was performed by two mechanisms, namely morphogenesis by the formation of hyphae and molecular basis by using RT-PCR. The frequency of C. albicans in the sample population was measured by calculating the percentage of samples that carry C. albicans. The frequency of C. albicans from HIV reached 40%, whereas in healthy people was only 15%. Molecular analysis of gene expression showed that isolates CH3 derived from HIV-positive patients with oral candidiasis could express SAP4-6 gene while commensal isolates from healthy people could not. Based on the results of this study it could be concluded that in terms of molecular detection only isolates derived from HIV-positive patients (CH3) could express its SAP4-6 gene.
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