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|Pohang University of Science and Technology, Korea|
|ScientificTracks Abstracts: J Cancer Sci Ther|
|Cancer stem cells (CSCs) are capable of tumour initiation and growth, and play a critical role in metastasis, therapeutic resistance, and disease recurrence in breast tumours. Therefore, if cancer arises and is maintained by the small population of CSCs within the bulk tumours, it is of central importance that definitive marker genes for CSCs are identified and regulatory mechanisms that promote stem cell maintenance be understood. Here, we show that the developmentally regulated HMG-box protein Capicua (CIC) is a transcriptional repressor that suppresses CSC properties in both the luminal and basal/myoepithelial subtypes of breast cancer cells. Mammosphere formation in culture was used to reveal stem cell properties, where expression of CIC was consistently down regulated in primary mammospheres in comparison to parental adherent cells then in secondary mammospheres upon serial passage. Knockdown of CIC increased mammosphere formation, while CIC overexpression prevented mammosphere formation, effect dependent on continuous CIC expression. Furthermore, CIC knockdown MCF7 and MDA-MB-231 breast cancer cells contained a higher percentage of EpCAM+/CD44+/CD24low/ cancer-initiating cells than in control cells grown as monolayer cultures and propagated as mammospheres. Loss of CIC relieved repression of PEA3 group genes, especially ETV4, which was necessary and sufficient for driving mammosphere formation. Moreover, we observed upregulation of the pluripotency-associated transcriptional factor SOX2 in MCF7 CIC knockdown cells and demonstrated significant rescue effect on mammosphere formation following SOX2 knockdown in CIC knockdown cells. Therefore, we propose CIC as a potential biomarker of breast cancer stem cells and a novel target in stem cell and cancer therapy.|
Jeehyun Yoe is a PhD candidate with particular interests in Stem Cell and Cancer Biology. Her current research has 2 aims: 1) to better understand the role of CIC in tumourigenic process in different cellular contexts and environments and 2) to further identify the molecular mechanisms that regulate CSC characteristics. Prior to enrolling at POSTECH for the combined MS and PhD program, she graduated with a BS in Biology and minors in Chemistry and Music from the University of North Carolina at Chapel Hill, USA.
Email: [email protected]
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