Cardioprotective effects of ÃŽÂ²-sitosterol from Linum usitatissimum against isoproterenol-induced myocardial infarction in rats: A biochemical, electrocardiographic and histological features

Amal Derbali; Kais Mnafgui; Abdelfattah Elfeki

doi:10.4172/2167-7689-C1-025

Cardioprotective effects of ÃÅ½ÃÂ²-sitosterol from Linum usitatissimum against isoproterenol-induced myocardial infarction in rats: A biochemical, electrocardiographic and histological features

9^th Annual European Pharma Congress

June 26-28, 2017 Madrid, Spain

Amal Derbali, Kais Mnafgui and Abdelfattah Elfeki

University of Sfax, Tunisia

Posters & Accepted Abstracts: Pharmaceut Reg Affairs

Abstract :

Myocardial infarction (heart attack) is the irreversible death (necrosis) of heart muscle secondary to prolonged lack of oxygen supply (ischemia). The present study was designed to evaluate cardioprotective effect of �?²-sitosterol, the major sterol of flaxseed oil, Linum usitatissimum, against isoproterenol induced myocardial infarction in rats. The research started with evaluating the sterol composition of Linum usitatissimum. Then, studying cardiovascular protective effect of its major sterol, �?²-sitosterol is based on cardiac damage markers especially electrocardiographic changes, histopathological modifications, troponin T and total cholesterol serum level. According to chemical analysis, this extract is composed essentially of stigmasterol (10.45%), avenasterol (13.30%), campesterol (25.33%) and �?²-sitosterol (44.08%). Male rats were randomly divided into four groups namely control (C), isoproterenol (ISO), isoproterenol treated group with clopidogrel (0.1 mg/kg body weight of clopidogrel/day) (CLO+ISO) and group treated with �?²-sitosterol (40 mg/kg body weight/day) (BS+ISO). Isoproterenol injection showed changes in electrocardiographic patterns, including ST-segment elevation. It caused the increase of the serum levels of troponin T and other cardiac injury biomarkers with antihypertensive effect through inhibition of angiotensin converting enzyme serum level. It also leaded to the appearance of edema and necrosis in myocardial tissue. However, �?²-sitosterol pre-co-treatment prevented almost all the parameters of isoproterenol-induced myocardial infarction in rats. To conclude, �?²-sitosterol, which is the active sterol of flaxseed oil, has a significant cardioprotective effects against isoproterenol-induced myocardial infarction.