Characterization of xylanase produced by Anoxybacillus sp. PD13 isolated from hot spring of Myagdi, Nepal

15^th Asia-Pacific Biotechnology Congress

July 20-22, 2017 Melbourne, Australia

Punam Yadav, Sikha Raj, Tribikram Bhattarai, Suresh Korpole, Gandham S Prasad, Girish Sahni, L Sreerama and Jyoti Maharjan

Nepal Academy of Science and Technology, Nepal
Microbial Type Culture Collection and Gene Bank, India
Tribhuvan University, Nepal
Qatar University, Qatar

Posters & Accepted Abstracts: J Bioprocess Biotech

Abstract :

NAST-PD13 isolated from Paudwar hot springs of Myagdi district, Nepal after morphological, biochemical and molecular identification suggested to be representative of the Anoxybacillus kamchatkensis (99.62% similarity) and was designated as Anoxybacillus kamchatkensis strain NAST PD13. Result shows that at pH 7, temperature 60 oC and in presence of organic nitrogen sources; NH4Cl, (NH4)2SO4 or yeast extract can produce optimum NAST-PD13 xylanase. Extracellular xylanase was precipitated by ammonium sulfate (80%) and purified using Sephadex G100 column chromatography. Molecular weight of xylanase was ~36.5 kDa as analyzed by Sodiumdodecyl Sulfate-Polyacrylamide Gel Electrophoreses (SDS-PAGE) and zymography. The optimum temperature and pH of purified enzyme were 60 oC and pH 9.0 respectively. Thin layer chromatography (TLC) shows that, the xylanase was capable of producing xylo-oligosaccharides. Further comparison with gene present show same molecular weight of our speculated xylanase and the annotated gene in NAST-PD13, which has its similarity (amino acid identity and molecular weight) to a predicted xylanase in other Anoxybacillus species. Thermal and alkaline pH activity of xylanase has potential applications in several industrial processes and this strain could be a good candidate for biotechnology and also source of novel industrial enzymes.

Biography :

Email: punamyadav.10oct@gmail.com