Chemical Modification Of Polyvinyl Alcohol Fibrous Carrier Support For Immobilization And Stabilization Of Alcohol Dehydrogenase | 67613
Journal of Medical Microbiology & Diagnosis
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Immobilization of yeast alcohol dehydrogenase (ADH) on polyvinyl alcohol fiber (PVA) carrier has been investigated with regards
to increase in stability at different pHs, storage and reusability. The strategy for immobilization involved functionalization of the
fibrous carrier with chloropropionyl chloride followed by amination with ethylenediamine (EDA) serving as a spacer and finally
crosslinking the ADH via glutaraldehyde (GA). The immobilization of ADH on PVA fibrous carrier results in optimal reaction
pH shift from 7 to 9 and improvement in thermal stability by preserving 80% activity upon heating at 60ºC for 2 h. In addition the
immobilized enzyme maintained 60% of its original activity after 8 repetitive use and showed reasonable storage stability at 4ºC in
phosphate buffer (PBS) for 28 days. Alcohol dehydrogenases (ADHs) are widely used enzyme from dehydrogenase group of the
enzyme to perform selective oxidations and reductions ranging from kinetic resolution to asymmetric reduction and can be used to
generate an asymmetric center to facilitate the synthesis of chiral drugs. Therefore there is an interest for stabilization of ADH which
in turn can be used in the development of flow reactors and biosensor applications.
Priydarshani Shinde has completed his MSc in Organic Chemistry from University of Pune and pursuing Doctoral research from Monash University in collaboration with National Science Agency of Australia (CSIRO), Melbourne, Australia. She has industrial experience in the biotech industry with a strong background of Biochemistry, Organic and Analytical Chemistry. She completed her Post-graduate Diploma in Patent Law. Currently, her research area extends the enhancement of ADH enzyme activity by immobilization methods for industrial applications.