alexa Chemical Modification Of Polyvinyl Alcohol Fibrous Carrier Support For Immobilization And Stabilization Of Alcohol Dehydrogenase
ISSN: 2161-0703

Journal of Medical Microbiology & Diagnosis
Open Access

Like us on:
OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Share This Page

Additional Info

Loading
Loading Please wait..
 

JOINT EVENT ON 15th International PHARMACEUTICAL MICROBIOLOGY AND BIOTECHNOLOGY CONFERENCE & 10th Annual MEDICAL MICROBIOLOGY SUMMIT & EXPO
June 21-23, 2017 London, UK

Priydarshani Shinde
Monash University, Australia
Posters & Accepted Abstracts: J Med Microb Diagn
DOI: 10.4172/2161-0703-C1-006
Abstract
Immobilization of yeast alcohol dehydrogenase (ADH) on polyvinyl alcohol fiber (PVA) carrier has been investigated with regards to increase in stability at different pHs, storage and reusability. The strategy for immobilization involved functionalization of the fibrous carrier with chloropropionyl chloride followed by amination with ethylenediamine (EDA) serving as a spacer and finally crosslinking the ADH via glutaraldehyde (GA). The immobilization of ADH on PVA fibrous carrier results in optimal reaction pH shift from 7 to 9 and improvement in thermal stability by preserving 80% activity upon heating at 60ºC for 2 h. In addition the immobilized enzyme maintained 60% of its original activity after 8 repetitive use and showed reasonable storage stability at 4ºC in phosphate buffer (PBS) for 28 days. Alcohol dehydrogenases (ADHs) are widely used enzyme from dehydrogenase group of the enzyme to perform selective oxidations and reductions ranging from kinetic resolution to asymmetric reduction and can be used to generate an asymmetric center to facilitate the synthesis of chiral drugs. Therefore there is an interest for stabilization of ADH which in turn can be used in the development of flow reactors and biosensor applications.
Biography

Priydarshani Shinde has completed his MSc in Organic Chemistry from University of Pune and pursuing Doctoral research from Monash University in collaboration with National Science Agency of Australia (CSIRO), Melbourne, Australia. She has industrial experience in the biotech industry with a strong background of Biochemistry, Organic and Analytical Chemistry. She completed her Post-graduate Diploma in Patent Law. Currently, her research area extends the enhancement of ADH enzyme activity by immobilization methods for industrial applications.

Email: [email protected]

image PDF   |   image HTML
 

Relevant Topics

Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri, Food, Aqua and Veterinary Science Journals

Dr. Krish

[email protected]

1-702-714-7001 Extn: 9040

Clinical and Biochemistry Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals

Ronald

[email protected]

1-702-714-7001Extn: 9042

Chemical Engineering and Chemistry Journals

Gabriel Shaw

[email protected]

1-702-714-7001 Extn: 9040

Earth & Environmental Sciences

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

Engineering Journals

James Franklin

[email protected]

1-702-714-7001Extn: 9042

General Science and Health care Journals

Andrea Jason

[email protected]

1-702-714-7001Extn: 9043

Genetics and Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001 Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Informatics Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Material Sciences Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Mathematics and Physics Journals

Jim Willison

[email protected]

1-702-714-7001 Extn: 9042

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001 Extn: 9038

Neuroscience & Psychology Journals

Nathan T

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

John Behannon

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001 Extn: 9042

 
© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
adwords