alexa CHO Glycosylation Mutant Cells As Potential Hosts For Production Of Therapeutic Biologics With Enhanced Efficacy | 71204
ISSN: 0975-0851

Journal of Bioequivalence & Bioavailability
Open Access

Like us on:

OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations
700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Joint Event: 3rd International Conference on Biopharmaceutics and Biologic Drugs & 5th International Pharmacy Conference

Zhiwei Song
Agency for Science, Technology and Research, Singapore
Posters & Accepted Abstracts: J Bioequiv Availab
DOI: 10.4172/0975-0851-C1-031
Abstract
Glycosylation can significantly affect the efficacy of recombinant therapeutics. Glycoprotein drugs require a high degree of sialylation of their N-glycans for a longer circulatory half-life. Mannose-terminated N-glycans can specifically target the proteins to macrophages and dendritic cells via mannose-binding receptors. Removal of core fucose from human IgG1 has been shown to significantly enhance its affinity to FcγRIIIa and thereby dramatically improves its antibody-dependent cellular cytotoxicity (ADCC). Cancer cells generally express glycoproteins with shortened O-glycans. Therefore, recombinant anti-cancer vaccines carrying these short tumor-associated O-glycans are more ideal for triggering specific anti-tumor immune responses. With cytotoxic lectins and the newly-developed genome editing technologies, such as ZFNs, TALENs and CRISPR-Cas9, we have generated more than 20 CHO glycosylation mutant cell lines. Some of these mutant lacks one specific glycosylation genes whereas others lack more than 10 glycosylation genes. With these CHO cell mutants, we have been able to produce highly sialylated EPO, recombinant human β-glucocerebrosidase with mannose-terminated N-glycans (like Cerezyme, but no need for in vitro glycan modification) and fucose-free antibodies. Furthermore, these mutant CHO cells can produce recombinant antibodies carrying different N-glycans with highly homogenous structures (90-97%). These are invaluable tools for antibody PK/PD studies on the impact of different N-glycans.
image PDF   |   image HTML
 

Relevant Topics

Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2018-19
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri & Aquaculture Journals

Dr. Krish

[email protected]

+1-702-714-7001Extn: 9040

Biochemistry Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals

Ronald

[email protected]

1-702-714-7001Extn: 9042

Chemistry Journals

Gabriel Shaw

[email protected]

1-702-714-7001Extn: 9040

Clinical Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Engineering Journals

James Franklin

[email protected]

1-702-714-7001Extn: 9042

Food & Nutrition Journals

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

General Science

Andrea Jason

[email protected]

1-702-714-7001Extn: 9043

Genetics & Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Materials Science Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Nursing & Health Care Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001Extn: 9038

Neuroscience & Psychology Journals

Nathan T

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

Ann Jose

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001Extn: 9042

 
© 2008- 2018 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version